21177-1-AP

antibody from Invitrogen Antibodies

Targeting: KIZ C20orf19, HT013, NCRNA00153, PLK1S1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

21177-1-AP

Provider

Invitrogen Antibodies

Product name

C20orf19 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: PVSEYCESE NKWSQEKHSP WEGVSDHLAH REPKSQKPFR KMQEEEEESW STSSDLTISI SEDDLILESP EPQPNPGGKM EGEDGIEALK LIHAEQERVA LSTEKNCILQ TLSSPDSEKE SSTNAPTREP GQTPDSDVPR AQVGQHVATL KEHDNSVKEE ATALLRKALT EECGRRSAIH SSESSCSLPS ILNDNSGIKE AKPAVWLNSV PTREQEVSSG CGDKSKKENV AADIPITETE AYQLLKKATL QDNTNQTENR FQKTDASVSH LSGLNIGSGA FETKTANKIA SEASFSSSEG SPLSRHENKK KPVINLKSNA LWDESDDSNS EIEAALRPRN HNTDDSDDFY D (324-673 aa encoded by BC045826)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.51 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 21177-1-AP (C20orf19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 21177-1-AP (C20orf19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using NCRNA00153 antibody 21177-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of 293 cells, using NCRNA00153 antibody 21177-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Kiz antibody (Product # 21177-1-AP) at dilution of 1:1200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using NCRNA00153 antibody 21177-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis using 21177-1-AP (C20orf19 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis using 21177-1-AP (C20orf19 antibody) at dilution of 1:100 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-C20orf19 (IP:21177-1-AP, 3ug; Detection:21177-1-AP 1:500) with HeLa cells lysate 2000ug.