PA5-118225

antibody from Invitrogen Antibodies

Targeting: PTRH2 BIT1, CFAP37, CGI-147, PTH2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118225

Provider

Invitrogen Antibodies

Product name

Bit1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Bit1 Polyclonal Antibody (Product # PA5-118225) at 1:2,000 dilution. Lane A: Jurkat Whole Cell Lysate, Lane B: HepG2 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 19 kDa. (We are unsure of the identity of these extra bands).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of Bit1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Bit1 Polyclonal Antibody (Product # PA5-118225, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human Bit1 in human kidney with Bit1 Polyclonal Antibody (Product # PA5-118225, 1:500 dilution, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human Bit1 in human liver with Bit1 Polyclonal Antibody (Product # PA5-118225, 1:500 dilution, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Bit1 Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 µL with Bit1 Polyclonal Antibody (Product # PA5-118225) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: Bit1 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 19 kDa.