14920-1-AP

antibody from Invitrogen Antibodies

Targeting: ATP6V1D ATP6M, VATD, VMA8

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14920-1-AP

Provider

Invitrogen Antibodies

Product name

ATP6V1D Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MSGKDRIEI FPSRMAQTIM KARLKGAQTG RNLLKKKSDA LTLRFRQILK KIIETKMLMG EVMREAAFSL AEAKFTAGDF STTVIQNVNK AQVKIRAKKD NVAGVTLPVF EHYHEGTDSY ELTGLARGGE QLAKLKRNYA KAVELLVELA SLQTSFVTLD EAIKITNRRV NAIEHVIIPR IERTLAYIIT ELDEREREEF YRLKKIQEKK KILKEKSEKD LEQRRAAGEV LEPANLLAEE KDEDLLFE (1-247 aa encoded by BC001411)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.35 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse lung tissue were subjected to SDS PAGE followed by western blot with 14920-1-AP (ATP6V1D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 14920-1-AP (ATP6V1D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer using 14920-1-AP (ATP6V1D antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer using 14920-1-AP (ATP6V1D antibody) at dilution of 1:100 (under 10x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HeLa cells were stained with 0.2ug ATP6V1D antibody (14920-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-ATP6V1D (IP:14920-1-AP, 4ug; Detection:14920-1-AP 1:500) with mouse lung tissue lysate 4000ug.