PA5-117802

antibody from Invitrogen Antibodies

Targeting: ATP6V1D ATP6M, VATD, VMA8

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117802

Provider

Invitrogen Antibodies

Product name

ATP6V1D Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ATP6V1D Polyclonal Antibody (Product # PA5-117802) at 1:500 dilution. Lane A: 293T Whole Cell Lysate, Lane B: HeLa Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 35 kDa. (We are unsure of the identity of these extra bands).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human ATP6V1D in human brain with ATP6V1D Polyclonal Antibody (Product # PA5-117802, 1:100 dilution, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human ATP6V1D in human kidney with ATP6V1D Polyclonal Antibody (Product # PA5-117802, 1:100 dilution, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ATP6V1D Immunoprecipitation using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 µL with ATP6V1D Polyclonal Antibody (Product # PA5-117802) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: ATP6V1D Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 38 kDa.