PA5-52994

antibody from Invitrogen Antibodies

Targeting: ASGR1 CLEC4H1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-52994

Provider

Invitrogen Antibodies

Product name

Anti-ASGR1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: RETFSNFTAS TEAQVKGLST QGGNVGRKMK SLESQLEKQQ KDLSEDHSSL LLHVKQFVSD LRSLSCQMAA LQGNGSERTC CPVNWVEHER SCYWFSRSGK AWADADNYCR LEDAHLVVVT SWE Highest antigen sequence identity to the following orthologs: Mouse - 76%, Rat - 76%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µl

Concentration

0.3 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ASGR1 in control (vector only transfected HEK293T lysate) and ASGR1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a ASGR1 Polyclonal Antibody (Product # PA5-52994).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ASGR1 in human liver tissue shows strong positivity in hepatocytes. Samples were probed using an ASGR1 Polyclonal Antibody (Product # PA5-52994).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ASGR1 in human liver and pancreas tissues using ASGR1 Polyclonal Antibody (Product # PA5-52994). Corresponding ASGR1 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ASGR1 in human colon, liver, lymph node and pancreas using ASGR1 Polyclonal Antibody (Product # PA5-52994) (A) shows similar protein distribution across tissues to an independent ASGR1 Polyclonal Antibody (B).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ASGR1 in human gastrointestinal using ASGR1 Polyclonal Antibody (Product # PA5-52994) shows no positivity in glandular cells as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ASGR1 in human lymphoid tissues using ASGR1 Polyclonal Antibody (Product # PA5-52994) shows no positivity in non-germinal center cells as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ASGR1 in human pancreas using ASGR1 Polyclonal Antibody (Product # PA5-52994) shows no positivity in exocrine glandular cells as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ASGR1 in human liver using ASGR1 Polyclonal Antibody (Product # PA5-52994) shows strong cytoplasmic positivity in hepatocytes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ASGR1 in human lymph node using ASGR1 Polyclonal Antibody (Product # PA5-52994).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ASGR1 in human colon using ASGR1 Polyclonal Antibody (Product # PA5-52994).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of ASGR1 in human cell line Hep G2 using an ASGR1 Polyclonal Antibody (Product # PA5-52994) shows localization to cell junctions and vesicles.