Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [10]
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Validation data
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- Product number
- NBP1-85581 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-85581, RRID:AB_11021923
- Product name
- Rabbit Polyclonal ASGPR1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human ASGPR1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Expression of asialoglycoprotein receptor 1 in human hepatocellular carcinoma.
Shi B, Abrams M, Sepp-Lorenzino L
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2013 Dec;61(12):901-9
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2013 Dec;61(12):901-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ASGPR1 Antibody [NBP1-85581] - Analysis in human cell line HepG2.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ASGPR1 Antibody [NBP1-85581] - Analysis in control (vector only transfected HEK293T lysate) and ASGR1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85581] - Staining of human liver shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85581] - Staining of human pancreas shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85581] - Staining of human colon.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85581] - Staining of human lymph node.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85581] - Staining of human tonsil shows no positivity in germinal center cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85581] - Staining in human liver and endometrium tissues using NBP1-85581 antibody. Corresponding ASGR1 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85581] - Staining of human endometrium shows no positivity in glandular cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85581] - Staining of human endometrium, kidney, liver and tonsil using Anti-ASGR1 antibody NBP1-85581 (A) shows similar protein distribution across tissues to independent antibody NBP1-85580 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85581] - Staining of human kidney shows no positivity in cells in tubules as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ASGPR1 Antibody [NBP1-85581] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.