Antibody data
- Antibody Data
- Antigen structure
- References [29]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
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Validation data
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- Product number
- H00001070-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001070-M01, RRID:AB_2082367
- Product name
- Mouse Monoclonal Centrin 3 Antibody
- Antibody type
- Monoclonal
- Description
- Centrin 3
- Reactivity
- Human, Zebrafish
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references New factors for protein transport identified by a genome-wide CRISPRi screen in mammalian cells.
Inhibition of CPAP-tubulin interaction prevents proliferation of centrosome-amplified cancer cells.
Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets.
Primary Cilia Mediate Diverse Kinase Inhibitor Resistance Mechanisms in Cancer.
Centrobin controls primary ciliogenesis in vertebrates.
Centriole triplet microtubules are required for stable centriole formation and inheritance in human cells.
Excess of a Rassf1-targeting microRNA, miR-193a-3p, perturbs cell division fidelity.
A CEP215-HSET complex links centrosomes with spindle poles and drives centrosome clustering in cancer.
Reduced levels of Dusp3/Vhr phosphatase impair normal spindle bipolarity in an Erk1/2 activity-dependent manner.
Severe NDE1-mediated microcephaly results from neural progenitor cell cycle arrests at multiple specific stages.
Opposing effects of pericentrin and microcephalin on the pericentriolar material regulate CHK1 activation in the DNA damage response.
Centrin 3 is an inhibitor of centrosomal Mps1 and antagonizes centrin 2 function.
CENP-W plays a role in maintaining bipolar spindle structure.
Cby1 promotes Ahi1 recruitment to a ring-shaped domain at the centriole-cilium interface and facilitates proper cilium formation and function.
Centmitor-1, a novel acridinyl-acetohydrazide, possesses similar molecular interaction field and antimitotic cellular phenotype as rigosertib, on 01910.Na.
Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy.
Calcium-binding capacity of centrin2 is required for linear POC5 assembly but not for nucleotide excision repair.
Abnormal centrosomal structure and duplication in Cep135-deficient vertebrate cells.
CEP162 is an axoneme-recognition protein promoting ciliary transition zone assembly at the cilia base.
DNA damage-induced centrosome amplification occurs via excessive formation of centriolar satellites.
FAM190A deficiency creates a cell division defect.
The Rilp-like proteins Rilpl1 and Rilpl2 regulate ciliary membrane content.
Loss of centrioles causes chromosomal instability in vertebrate somatic cells.
Disruption of mouse Cenpj, a regulator of centriole biogenesis, phenocopies Seckel syndrome.
C-NAP1 and rootletin restrain DNA damage-induced centriole splitting and facilitate ciliogenesis.
A primary microcephaly protein complex forms a ring around parental centrioles.
Defective nucleotide excision repair with normal centrosome structures and functions in the absence of all vertebrate centrins.
TPCK targets elements of mitotic spindle and induces cell cycle arrest in prometaphase.
CDK5RAP2 functions in centrosome to spindle pole attachment and DNA damage response.
Bassaganyas L, Popa SJ, Horlbeck M, Puri C, Stewart SE, Campelo F, Ashok A, Butnaru CM, Brouwers N, Heydari K, Ripoche J, Weissman J, Rubinsztein DC, Schekman R, Malhotra V, Moreau K, Villeneuve J
The Journal of cell biology 2019 Nov 4;218(11):3861-3879
The Journal of cell biology 2019 Nov 4;218(11):3861-3879
Inhibition of CPAP-tubulin interaction prevents proliferation of centrosome-amplified cancer cells.
Mariappan A, Soni K, Schorpp K, Zhao F, Minakar A, Zheng X, Mandad S, Macheleidt I, Ramani A, Kubelka T, Dawidowski M, Golfmann K, Wason A, Yang C, Simons J, Schmalz HG, Hyman AA, Aneja R, Ullrich R, Urlaub H, Odenthal M, Büttner R, Li H, Sattler M, Hadian K, Gopalakrishnan J
The EMBO journal 2019 Jan 15;38(2)
The EMBO journal 2019 Jan 15;38(2)
Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets.
Krastev DB, Pettitt SJ, Campbell J, Song F, Tanos BE, Stoynov SS, Ashworth A, Lord CJ
Nature communications 2018 May 22;9(1):2016
Nature communications 2018 May 22;9(1):2016
Primary Cilia Mediate Diverse Kinase Inhibitor Resistance Mechanisms in Cancer.
Jenks AD, Vyse S, Wong JP, Kostaras E, Keller D, Burgoyne T, Shoemark A, Tsalikis A, de la Roche M, Michaelis M, Cinatl J Jr, Huang PH, Tanos BE
Cell reports 2018 Jun 5;23(10):3042-3055
Cell reports 2018 Jun 5;23(10):3042-3055
Centrobin controls primary ciliogenesis in vertebrates.
Ogungbenro YA, Tena TC, Gaboriau D, Lalor P, Dockery P, Philipp M, Morrison CG
The Journal of cell biology 2018 Apr 2;217(4):1205-1215
The Journal of cell biology 2018 Apr 2;217(4):1205-1215
Centriole triplet microtubules are required for stable centriole formation and inheritance in human cells.
Wang JT, Kong D, Hoerner CR, Loncarek J, Stearns T
eLife 2017 Sep 14;6
eLife 2017 Sep 14;6
Excess of a Rassf1-targeting microRNA, miR-193a-3p, perturbs cell division fidelity.
Pruikkonen S, Kallio MJ
British journal of cancer 2017 May 23;116(11):1451-1461
British journal of cancer 2017 May 23;116(11):1451-1461
A CEP215-HSET complex links centrosomes with spindle poles and drives centrosome clustering in cancer.
Chavali PL, Chandrasekaran G, Barr AR, Tátrai P, Taylor C, Papachristou EK, Woods CG, Chavali S, Gergely F
Nature communications 2016 Mar 18;7:11005
Nature communications 2016 Mar 18;7:11005
Reduced levels of Dusp3/Vhr phosphatase impair normal spindle bipolarity in an Erk1/2 activity-dependent manner.
Tambe MB, Narvi E, Kallio M
FEBS letters 2016 Aug;590(16):2757-67
FEBS letters 2016 Aug;590(16):2757-67
Severe NDE1-mediated microcephaly results from neural progenitor cell cycle arrests at multiple specific stages.
Doobin DJ, Kemal S, Dantas TJ, Vallee RB
Nature communications 2016 Aug 24;7:12551
Nature communications 2016 Aug 24;7:12551
Opposing effects of pericentrin and microcephalin on the pericentriolar material regulate CHK1 activation in the DNA damage response.
Antonczak AK, Mullee LI, Wang Y, Comartin D, Inoue T, Pelletier L, Morrison CG
Oncogene 2016 Apr 14;35(15):2003-10
Oncogene 2016 Apr 14;35(15):2003-10
Centrin 3 is an inhibitor of centrosomal Mps1 and antagonizes centrin 2 function.
Sawant DB, Majumder S, Perkins JL, Yang CH, Eyers PA, Fisk HA
Molecular biology of the cell 2015 Nov 1;26(21):3741-53
Molecular biology of the cell 2015 Nov 1;26(21):3741-53
CENP-W plays a role in maintaining bipolar spindle structure.
Kaczmarczyk A, Sullivan KF
PloS one 2014;9(10):e106464
PloS one 2014;9(10):e106464
Cby1 promotes Ahi1 recruitment to a ring-shaped domain at the centriole-cilium interface and facilitates proper cilium formation and function.
Lee YL, Santé J, Comerci CJ, Cyge B, Menezes LF, Li FQ, Germino GG, Moerner WE, Takemaru K, Stearns T
Molecular biology of the cell 2014 Oct 1;25(19):2919-33
Molecular biology of the cell 2014 Oct 1;25(19):2919-33
Centmitor-1, a novel acridinyl-acetohydrazide, possesses similar molecular interaction field and antimitotic cellular phenotype as rigosertib, on 01910.Na.
Mäki-Jouppila JH, Laine LJ, Rehnberg J, Narvi E, Tiikkainen P, Hukasova E, Halonen P, Lindqvist A, Kallio L, Poso A, Kallio MJ
Molecular cancer therapeutics 2014 May;13(5):1054-66
Molecular cancer therapeutics 2014 May;13(5):1054-66
Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy.
Martin CA, Ahmad I, Klingseisen A, Hussain MS, Bicknell LS, Leitch A, Nürnberg G, Toliat MR, Murray JE, Hunt D, Khan F, Ali Z, Tinschert S, Ding J, Keith C, Harley ME, Heyn P, Müller R, Hoffmann I, Cormier-Daire V, Dollfus H, Dupuis L, Bashamboo A, McElreavey K, Kariminejad A, Mendoza-Londono R, Moore AT, Saggar A, Schlechter C, Weleber R, Thiele H, Altmüller J, Höhne W, Hurles ME, Noegel AA, Baig SM, Nürnberg P, Jackson AP
Nature genetics 2014 Dec;46(12):1283-1292
Nature genetics 2014 Dec;46(12):1283-1292
Calcium-binding capacity of centrin2 is required for linear POC5 assembly but not for nucleotide excision repair.
Dantas TJ, Daly OM, Conroy PC, Tomas M, Wang Y, Lalor P, Dockery P, Ferrando-May E, Morrison CG
PloS one 2013;8(7):e68487
PloS one 2013;8(7):e68487
Abnormal centrosomal structure and duplication in Cep135-deficient vertebrate cells.
Inanç B, Pütz M, Lalor P, Dockery P, Kuriyama R, Gergely F, Morrison CG
Molecular biology of the cell 2013 Sep;24(17):2645-54
Molecular biology of the cell 2013 Sep;24(17):2645-54
CEP162 is an axoneme-recognition protein promoting ciliary transition zone assembly at the cilia base.
Wang WJ, Tay HG, Soni R, Perumal GS, Goll MG, Macaluso FP, Asara JM, Amack JD, Tsou MF
Nature cell biology 2013 Jun;15(6):591-601
Nature cell biology 2013 Jun;15(6):591-601
DNA damage-induced centrosome amplification occurs via excessive formation of centriolar satellites.
Löffler H, Fechter A, Liu FY, Poppelreuther S, Krämer A
Oncogene 2013 Jun 13;32(24):2963-72
Oncogene 2013 Jun 13;32(24):2963-72
FAM190A deficiency creates a cell division defect.
Patel K, Scrimieri F, Ghosh S, Zhong J, Kim MS, Ren YR, Morgan RA, Iacobuzio-Donahue CA, Pandey A, Kern SE
The American journal of pathology 2013 Jul;183(1):296-303
The American journal of pathology 2013 Jul;183(1):296-303
The Rilp-like proteins Rilpl1 and Rilpl2 regulate ciliary membrane content.
Schaub JR, Stearns T
Molecular biology of the cell 2013 Feb;24(4):453-64
Molecular biology of the cell 2013 Feb;24(4):453-64
Loss of centrioles causes chromosomal instability in vertebrate somatic cells.
Sir JH, Pütz M, Daly O, Morrison CG, Dunning M, Kilmartin JV, Gergely F
The Journal of cell biology 2013 Dec 9;203(5):747-56
The Journal of cell biology 2013 Dec 9;203(5):747-56
Disruption of mouse Cenpj, a regulator of centriole biogenesis, phenocopies Seckel syndrome.
McIntyre RE, Lakshminarasimhan Chavali P, Ismail O, Carragher DM, Sanchez-Andrade G, Forment JV, Fu B, Del Castillo Velasco-Herrera M, Edwards A, van der Weyden L, Yang F, Sanger Mouse Genetics Project., Ramirez-Solis R, Estabel J, Gallagher FA, Logan DW, Arends MJ, Tsang SH, Mahajan VB, Scudamore CL, White JK, Jackson SP, Gergely F, Adams DJ
PLoS genetics 2012;8(11):e1003022
PLoS genetics 2012;8(11):e1003022
C-NAP1 and rootletin restrain DNA damage-induced centriole splitting and facilitate ciliogenesis.
Conroy PC, Saladino C, Dantas TJ, Lalor P, Dockery P, Morrison CG
Cell cycle (Georgetown, Tex.) 2012 Oct 15;11(20):3769-78
Cell cycle (Georgetown, Tex.) 2012 Oct 15;11(20):3769-78
A primary microcephaly protein complex forms a ring around parental centrioles.
Sir JH, Barr AR, Nicholas AK, Carvalho OP, Khurshid M, Sossick A, Reichelt S, D'Santos C, Woods CG, Gergely F
Nature genetics 2011 Oct 9;43(11):1147-53
Nature genetics 2011 Oct 9;43(11):1147-53
Defective nucleotide excision repair with normal centrosome structures and functions in the absence of all vertebrate centrins.
Dantas TJ, Wang Y, Lalor P, Dockery P, Morrison CG
The Journal of cell biology 2011 Apr 18;193(2):307-18
The Journal of cell biology 2011 Apr 18;193(2):307-18
TPCK targets elements of mitotic spindle and induces cell cycle arrest in prometaphase.
Fabian Z, Fearnhead HO
Biochemical and biophysical research communications 2010 May 14;395(4):458-64
Biochemical and biophysical research communications 2010 May 14;395(4):458-64
CDK5RAP2 functions in centrosome to spindle pole attachment and DNA damage response.
Barr AR, Kilmartin JV, Gergely F
The Journal of cell biology 2010 Apr 5;189(1):23-39
The Journal of cell biology 2010 Apr 5;189(1):23-39
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Centrin 3 Antibody (3E6) [H00001070-M01] - Analysis of CETN3 expression in transfected 293T cell line by CETN3 monoclonal antibody (M01), clone 3E6.Lane 1: CETN3 transfected lysate(19.5 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Centrin 3 Antibody (3E6) [H00001070-M01] - CETN3 monoclonal antibody (M01), clone 3E6 Analysis of CETN3 expression in Jurkat.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Centrin 3 Antibody (3E6.) [H00001070-M01] - Centrin 3 Antibody (3E6) [H00001070-M01] - Analysis of CETN3 over-expressed 293 cell line, cotransfected with CETN3 Validated Chimera RNAi ( Cat # H00001070-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CETN3 monoclonal antibody (M01), clone 3E6 (Cat # H00001070-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Centrin 3 Antibody (3E6.) [H00001070-M01] - Centrin 3 Antibody (3E6) [H00001070-M01] - Detection limit for recombinant GST tagged CETN3 is approximately 0.1ng/ml as a capture antibody.