PA5-96938

antibody from Invitrogen Antibodies

Targeting: CETN3 CEN3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96938

Provider

Invitrogen Antibodies

Product name

Centrin 3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MSLALRSELV VDKTKRKKRR ELSEEQKQEI KDAFELFDTD KDEAIDYHEL KVAMRALGFD VKKADVLKIL KDYDREATGK ITFEDFNEVV TDWILERDPH EEILKAFKLF DDDDSGKISL RNLRRVAREL GENMSDEELR AMIEEFDKDG DGEINQEEFI AIMTGDI; Positive Samples: MCF-7, Jurkat, 293T, Mouse testis, Mouse spleen, Mouse lung, Mouse ovary; Cellular Location: Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, nucleolus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.97 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using CETN3 Polyclonal antibody (Product # PA5-96938) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Centrin 3 in extracts of various cell lines using Centrin 3 Polyclonal Antibody (Product # PA5-96938) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Centrin 3 was performed in C6 cells using Centrin 3 Polyclonal Antibody (Product # PA5-96938) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Centrin 3 was performed in C6 cells using Centrin 3 Polyclonal Antibody (Product # PA5-96938) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Centrin 3 was performed in L929 cells using Centrin 3 Polyclonal Antibody (Product # PA5-96938) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Centrin 3 was performed in U2OS cells using Centrin 3 Polyclonal Antibody (Product # PA5-96938) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Centrin 3 in paraffin-embedded human liver damage using Centrin 3 Polyclonal Antibody (Product # PA5-96938) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Centrin 3 in paraffin-embedded human lung cancer using Centrin 3 Polyclonal Antibody (Product # PA5-96938) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Centrin 3 in paraffin-embedded human stomach using Centrin 3 Polyclonal Antibody (Product # PA5-96938) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Centrin 3 in paraffin-embedded rat brain using Centrin 3 Polyclonal Antibody (Product # PA5-96938) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Centrin 3 in paraffin-embedded mouse kidney using Centrin 3 Polyclonal Antibody (Product # PA5-96938) at a dilution of 1:100.