PA5-109324

antibody from Invitrogen Antibodies

Targeting: KCNJ11 BIR, Kir6.2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-109324

Provider

Invitrogen Antibodies

Product name

Kir6.2 (KCNJ11) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: Human liver tissue, mouse liver tissue, Lovo, mouse pancreas tissue, human prostate cancer tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Kir6.2 in Positive control: Lane 1: Human liver tissue lysate, Lane 2: Mouse liver tissue lysate. Samples were transferred to PVDF membrane, blocked with 5% BSA (1 hour), incubated with Kir6.2 polyclonal antibody (Product # PA5-109324), at a dilution of 1:500 (5% BSA, 2 hours), followed by Goat Anti-Rabbit IgG-HRP (1 hour) with a dilution of 1:5000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Kir6.2 in LoVo cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with Kir6.2 polyclonal antibody (Product # PA5-109324) at a dilution of 1:200 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:100.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Kir6.2 in paraffin-embedded mouse pancreas tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with Kir6.2 polyclonal antibody (Product # PA5-109324) at a dilution of 1:200 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Kir6.2 in paraffin-embedded mouse liver tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with Kir6.2 polyclonal antibody (Product # PA5-109324) at a dilution of 1:50 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Kir6.2 in paraffin-embedded human prostate cancer tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with Kir6.2 polyclonal antibody (Product # PA5-109324) at a dilution of 1:50 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).