A00895-1

antibody from Boster Biological Technology

Targeting: ABCC8 ABC36, HHF1, HI, HRINS, MRP8, PHHI, SUR, SUR1, TNDM2

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

A00895-1

Provider

Boster Biological Technology

Product name

Anti-SUR1/ABCC8 Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for SUR1/ABCC8 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: Flow Cytometry. Reactive species: Human. SUR1/ABCC8 information: Subcellular Localization: Cell membrane.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Flow cytometry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Flow Cytometry analysis of A431 cells using anti-ABCC8 antibody (A00895-1). Overlay histogram showing A431 cells stained with A00895-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCC8 Antibody (A00895-1,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Additional image