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- Antibody Data
- Antigen structure
- References [13]
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- Western blot [2]
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- Product number
- NB300-147 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-147, RRID:AB_2060999
- Product name
- Mouse Monoclonal Sodium Potassium ATPase Beta 1 Antibody
- Antibody type
- Monoclonal
- Description
- Unpurified. This is specific for Na,K-ATPase beta 1 subunit.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Hamster, Porcine, Rabbit, Xenopus
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Insulin acutely increases glucose transporter 1 on plasma membranes and glucose uptake in an AKT-dependent manner in chicken adipocytes.
The O-glycosylated ectodomain of FXYD5 impairs adhesion by disrupting cell-cell trans-dimerization of Na,K-ATPase β1 subunits.
Septin dynamics are essential for exocytosis.
Na+/K+-ATPase β1-subunit is recruited in Na-K-2Cl co-transporter isoform 2 multiprotein complexes in rat kidneys: possible role in blood pressure regulation.
Protective effect of truncated Na+/K+-ATPase β on ischemia/reperfusion-induced renal injury in rats.
Identification of the amino acid region involved in the intercellular interaction between the β1 subunits of Na+/K+ -ATPase.
Glucose and collagen regulate human platelet activity through aldose reductase induction of thromboxane.
Expression of H(+),K(+)-ATPase and glycopattern analysis in the gastric glands of Rana esculenta.
Distinct endocytic recycling of myelin proteins promotes oligodendroglial membrane remodeling.
The C-terminal flavin domain of gp91phox bound to plasma membranes of granulocyte-like X-CGD PLB-985 cells is sufficient to anchor cytosolic oxidase components and support NADPH oxidase-associated diaphorase activity independent of cytosolic phospholipase A2 regulation.
Recombinant addition of N-glycosylation sites to the basolateral Na,K-ATPase beta1 subunit results in its clustering in caveolae and apical sorting in HGT-1 cells.
Use of the H,K-ATPase beta subunit to identify multiple sorting pathways for plasma membrane delivery in polarized cells.
The H,K-ATPase beta subunit as a model to study the role of N-glycosylation in membrane trafficking and apical sorting.
Shimamoto S, Nakashima K, Kamimura R, Kohrogi R, Inoue H, Nishikoba N, Ohtsuka A, Ijiri D
General and comparative endocrinology 2019 Nov 1;283:113232
General and comparative endocrinology 2019 Nov 1;283:113232
The O-glycosylated ectodomain of FXYD5 impairs adhesion by disrupting cell-cell trans-dimerization of Na,K-ATPase β1 subunits.
Tokhtaeva E, Sun H, Deiss-Yehiely N, Wen Y, Soni PN, Gabrielli NM, Marcus EA, Ridge KM, Sachs G, Vazquez-Levin M, Sznajder JI, Vagin O, Dada LA
Journal of cell science 2016 Jun 15;129(12):2394-406
Journal of cell science 2016 Jun 15;129(12):2394-406
Septin dynamics are essential for exocytosis.
Tokhtaeva E, Capri J, Marcus EA, Whitelegge JP, Khuzakhmetova V, Bukharaeva E, Deiss-Yehiely N, Dada LA, Sachs G, Fernandez-Salas E, Vagin O
The Journal of biological chemistry 2015 Feb 27;290(9):5280-97
The Journal of biological chemistry 2015 Feb 27;290(9):5280-97
Na+/K+-ATPase β1-subunit is recruited in Na-K-2Cl co-transporter isoform 2 multiprotein complexes in rat kidneys: possible role in blood pressure regulation.
Carmosino M, Torretta S, Procino G, Timperio A, Zolla L, Svelto M
Journal of hypertension 2014 Sep;32(9):1842-53
Journal of hypertension 2014 Sep;32(9):1842-53
Protective effect of truncated Na+/K+-ATPase β on ischemia/reperfusion-induced renal injury in rats.
Gong H, Sun J, Xue W, Tian P, Ding X, Yan H, Li Y, Zheng J
Experimental biology and medicine (Maywood, N.J.) 2014 Jun;239(6):677-85
Experimental biology and medicine (Maywood, N.J.) 2014 Jun;239(6):677-85
Identification of the amino acid region involved in the intercellular interaction between the β1 subunits of Na+/K+ -ATPase.
Tokhtaeva E, Sachs G, Sun H, Dada LA, Sznajder JI, Vagin O
Journal of cell science 2012 Mar 15;125(Pt 6):1605-16
Journal of cell science 2012 Mar 15;125(Pt 6):1605-16
Glucose and collagen regulate human platelet activity through aldose reductase induction of thromboxane.
Tang WH, Stitham J, Gleim S, Di Febbo C, Porreca E, Fava C, Tacconelli S, Capone M, Evangelista V, Levantesi G, Wen L, Martin K, Minuz P, Rade J, Patrignani P, Hwa J
The Journal of clinical investigation 2011 Nov;121(11):4462-76
The Journal of clinical investigation 2011 Nov;121(11):4462-76
Expression of H(+),K(+)-ATPase and glycopattern analysis in the gastric glands of Rana esculenta.
Mastrodonato M, Calamita G, Rossi R, Scillitani G, Liquori GE, Ferri D
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2009 Mar;57(3):215-25
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2009 Mar;57(3):215-25
Distinct endocytic recycling of myelin proteins promotes oligodendroglial membrane remodeling.
Winterstein C, Trotter J, Krämer-Albers EM
Journal of cell science 2008 Mar 15;121(Pt 6):834-42
Journal of cell science 2008 Mar 15;121(Pt 6):834-42
The C-terminal flavin domain of gp91phox bound to plasma membranes of granulocyte-like X-CGD PLB-985 cells is sufficient to anchor cytosolic oxidase components and support NADPH oxidase-associated diaphorase activity independent of cytosolic phospholipase A2 regulation.
Pessach I, Shmelzer Z, Leto TL, Dinauer MC, Levy R
Journal of leukocyte biology 2006 Sep;80(3):630-9
Journal of leukocyte biology 2006 Sep;80(3):630-9
Recombinant addition of N-glycosylation sites to the basolateral Na,K-ATPase beta1 subunit results in its clustering in caveolae and apical sorting in HGT-1 cells.
Vagin O, Turdikulova S, Sachs G
The Journal of biological chemistry 2005 Dec 30;280(52):43159-67
The Journal of biological chemistry 2005 Dec 30;280(52):43159-67
Use of the H,K-ATPase beta subunit to identify multiple sorting pathways for plasma membrane delivery in polarized cells.
Vagin O, Turdikulova S, Yakubov I, Sachs G
The Journal of biological chemistry 2005 Apr 15;280(15):14741-54
The Journal of biological chemistry 2005 Apr 15;280(15):14741-54
The H,K-ATPase beta subunit as a model to study the role of N-glycosylation in membrane trafficking and apical sorting.
Vagin O, Turdikulova S, Sachs G
The Journal of biological chemistry 2004 Sep 10;279(37):39026-34
The Journal of biological chemistry 2004 Sep 10;279(37):39026-34
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Western blot analysis of Sodium Potassium ATPase Beta 1 expression in 1) SK-BR-3 and 2) A-431 whole cell lysates using NB300-147.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Detection of Na, K-ATPase (beta) in rat kidney homogenates (20 ug)using NB 300-147. Lane 1: 1:2,500 primary. Lane 2: 1:5,000 primary.
