MA3-930

antibody from Invitrogen Antibodies

Targeting: ATP1B1 ATP1B

Provider product page for MA3-930

Western blot

Immunocytochemistry

Immunohistochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [55]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [3]
Immunohistochemistry [3]
Other assay [22]

Submit

Product number: MA3-930 - Provider product page
Provider: Invitrogen Antibodies
Product name: ATP1B1 Monoclonal Antibody (M17-P5-F11)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA3-930 detects sodium/potassium ATPase beta 1 from mouse, human, sheep, canine, and pig tissues. This antibody does not recognize the beta 1 or beta 2 subunit in rat.
Antibody clone number: M17-P5-F11
Concentration: 1 mg/mL

ATP1B1 protein structure - MA3-930 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Sodiµm/Potassium ATPase beta using Anti-Sodiµm/Potassium ATPase beta Monoclonal Antibody (M17-P5-F11) (Product # MA3-930) shows staining in Hela Cells. Sodiµm/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodiµm/Potassium ATPase beta (Product # MA3-930) at a dilution of 1:200 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Sodiµm/Potassium ATPase beta using Anti-Sodiµm/Potassium ATPase beta Monoclonal Antibody (M17-P5-F11) (Product # MA3-930) shows staining in MCF-7 Cells. Sodiµm/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodiµm/Potassium ATPase beta (Product # MA3-930) at a dilution of 1:200 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Sodiµm/Potassium ATPase beta using Anti-Sodiµm/Potassium ATPase beta Monoclonal Antibody (M17-P5-F11) (Product # MA3-930) shows staining in U251 Cells. Sodiµm/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodiµm/Potassium ATPase beta (Product # MA3-930) at a dilution of 1:200 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry was performed on cancer biopsies of deparaffinized Human colon carcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodiµm/Potassium ATPase beta (Product # MA3-930) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry was performed on normal deparaffinized Human liver tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodiµm/Potassium ATPase beta (Product # MA3-930) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry was performed on normal deparaffinized Human tonsil tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodiµm/Potassium ATPase beta (Product # MA3-930) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 The T 12 GT 3 GT 6 allele regulates the polyadenylation of ATP1B1 mRNA in human tissues A) Schematic of the primer design used to distinguish between the A2- and A5- polyadenylated mRNAs. The A2-R primer had a 5' T 15 tail to selectively amplify A2-polyadenylated mRNA. Total ATP1B1 mRNA was measured using primers that amplified across the exon 5/exon 6 junction. B) Real-time PCR of mRNA from human kidneys. The A2-polyadenylated transcript was quantified relative to total ATP1B1 mRNA. 0 and 1 indicate subjects without the T 12 GT 3 GT 6 allele and with 1 copy of the T 12 GT 3 GT 6 allele, respectively. Error bars represent standard errors. * p < 0.0001. C) Real-time PCR of mRNA from human lymphocytes. Expression of the A2- and A5-polyadenylated mRNAs relative to total ATP1B1 mRNA in lymphocytes from subjects with 0 or at least 1 copy of the T 12 GT 3 GT 6 allele. * p = 0.0001 between the A5-polyadenylated transcript levels in the 2 groups. Data represent the average across samples from each genotype group with standard error. D) Expression of ATP1B1 protein in lymphocytes from individuals with 0 or at least 1 copy of the T 12 GT 3 GT 6 allele. Tubulin was used as a loading control. The graph quantifies the average level of ATP1B1 protein relative to tubulin with standard error. * p = 0.05.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Representative western blot images for each NKA isoform analyzed ( alpha 1-3 and beta 1-2 ), including example of the coomassie stain used to normalize blot density to the total protein content of the sample. ""Inj"" represents the injured leg from the KI group, ""Non"" represents the noninjured leg from the KI group, and ""CON"" represents the control group.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 2 Confocal immunofluorescence of Na/K-ATPase (beta1 isoform) and Claudin-11 in the lateral wall of the basal and apical (inset) turns. Claudin-11 is expressed basally from the superior epithelium of the SP to the suprastrial space insulating the K + secreting marginal cell layer. Non-polarized beta1 Na/K-ATPase activity is high in type II and type V fibrocytes conceivably reflecting the uptake and recirculation of K + . RM, Reissner's membrane.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 4 Confocal and super-resolution (SIM) microscopy of the suprastrial space (SS). (A) Insulating type V fibrocytes express Claudin-11. The left inset shows cells facing the scala vestibuli (arrows). (B) Cx30 expression also reaches the SS reflecting conceivable transcellular recirculation of K + from SV back to the StV. Laminin beta2 stains the basal lamina of the RM and capillaries. (C) Polarized expression of NKCC1 and Na/K-ATPase (beta1) in the StV and non-polarized manifestation in the suprastrial space may reflect the alternate directions of ion flow. Framed area is magnified in (D,E) and show expression of the NKCC1 and Na/K-ATPase (beta1) membrane transporters facing SV. (F) The type V fibrocyte facing the SV shows both intracellular ( * ) and membrane expression (arrow) of Na/K-ATPase (SR-SIM). StV, stria vascularis; RM, Reissner's membrane; V, stria vessel; SV, scala vestibuli.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blotting analysis showing protein expression of TXNRD1, HSPA4L and ATP1B1 in ( A ) the fresh pre-freeze (PF) and ( B ) frozen-thawed (FT) sperm from boars of the good and poor semen freezability (GSF and PSF, respectively) groups.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Muscle NKA alpha 1 (A), alpha 2 (B), and alpha 3 (C) isoform relative abundance from the vastus lateralis of the injured and noninjured legs of participants with knee injury (KI) and from a single leg of age- and BMI-matched controls (CON). Representative western blots are included; Inj represents the knee-injured leg, Non-I represents the noninjured leg, and CON represents the control group. Unfilled bars represent the injured leg from KI, filled bars represent the noninjured leg from KI and the hatched bars represent CON. *Less than noninjured leg ( P < 0.05), &ddagger; Moderate effect size compared to noninjured leg. Values are Mean +- SD, n = 6 for each leg KI, n = 7 CON.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Muscle NKA beta 1 (A) and beta 2 (B) isoform relative abundance from the vastus lateralis of the injured and noninjured legs of participants with knee injury (KI) and from a single leg of age- and BMI-matched controls (CON). Representative western blots are included; ""Inj"" represents the knee-injured leg, ""Non-I"" represents the noninjured leg, and ""CON ""represents the control group. Hollow bars represent the injured leg from KI, filled bars represent the noninjured leg from KI and the hatched bars represent the CON. &ddagger; Moderate effect size compared to noninjured leg in KI. Values are Mean +- SD, n = 6 for each leg KI, n = 7 CON.