Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-C348695 - Provider product page
- Provider
- LSBio
- Product name
- Anti-DAGLB Antibody LS-C348695
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Rat
- Host
- Rabbit
- Vial size
- 100µl
- Storage
- Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoblot of DAGL-alpha using antibody at 1:100 in 40 ug of rat brain membranes, developed using the ECL technique.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemical detection of DAGL-alpha on formaldehyde fixed rat ependyma slices. Antigen retrieval: 30 min incubation at 80ºC in 50 mM sodium citrate pH 9. 10% donkey serum as blocking agent for 1 hour Primary antibody dilution 1:50 overnight Secondary antibody: anti-rabbit biotin-conjugated IgG (1/500) (Suárez et al., 2010).
