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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB2941 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2941, RRID:AB_1577008
- Product name
- MAK polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of MAK.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Identification of human male germ cell-associated kinase, a kinase transcriptionally activated by androgen in prostate cancer cells.
Mouse Mak gene for male germ cell-associated kinase maps to chromosome 13.
Testis-specific mak protein kinase is expressed specifically in the meiotic phase in spermatogenesis and is associated with a 210-kilodalton cellular phosphoprotein.
Xia L, Robinson D, Ma AH, Chen HC, Wu F, Qiu Y, Kung HJ
The Journal of biological chemistry 2002 Sep 20;277(38):35422-33
The Journal of biological chemistry 2002 Sep 20;277(38):35422-33
Mouse Mak gene for male germ cell-associated kinase maps to chromosome 13.
Taketo M, Jinno A, Yamaguchi S, Matushime H, Shibuya M, Seldin MF
Genomics 1994 Jan 15;19(2):397-8
Genomics 1994 Jan 15;19(2):397-8
Testis-specific mak protein kinase is expressed specifically in the meiotic phase in spermatogenesis and is associated with a 210-kilodalton cellular phosphoprotein.
Jinno A, Tanaka K, Matsushime H, Haneji T, Shibuya M
Molecular and cellular biology 1993 Jul;13(7):4146-56
Molecular and cellular biology 1993 Jul;13(7):4146-56
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of MAK polyclonal antibody (Cat # PAB2941) in HL-60 cell lysate (Lane 1) and mouse brain tissue lysate (Lane 2) . MAK (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with MAK polyclonal antibody (Cat # PAB2941) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
