PA5-112076

antibody from Invitrogen Antibodies

Targeting: GCM2 GCMB, hGCMb

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-112076

Provider

Invitrogen Antibodies

Product name

GCM2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of GCM2 using GCM2 Polyclonal Antibody (Product # PA5-112076) at a dilution of 1:500. Lane A: A431 whole cell lysate, (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Rabbit IgG H&L (Dylight 800) at 1:10,000 dilution. Developed using the Odyssey technique and performed under reducing conditions. Predicted band size: 56 kDa. Observed band size: 56 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of GCM2 in A431 cells. Cells were fixed with 4% PFA, permeabilized with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with GCM2 Polyclonal Antibody (Product # PA5-112076) (1:5000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.