M04526

antibody from Boster Biological Technology

Targeting: RAB11B H-YPT3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M04526

Provider

Boster Biological Technology

Product name

Anti-RAB11B Antibody Picoband™ (monoclonal, 6C5)

Antibody type

Monoclonal

Description

Mouse IgG monoclonal antibody for RAB11B detection. Tested with WB, ICC/IF, FCM in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

6C5

Vial size

100μg/vial

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of RAB11B using anti- RAB11B antibody (M04526). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat lung whole cell lysates, Lane 3: rat spleen whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain whole cell lysates. Lane 6: mouse lung whole cell lysates. Lane 7: mouse spleen whole cell lysates. Lane 8: mouse Neuro-2a whole cell lysates. Lane 9: mouse RAW246.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-RAB11B antigen affinity purified polyclonal antibody (Catalog # M04526) at 0.5 g/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for RAB11B at approximately 24KD. The expected band size for RAB11B is at 24KD.

Additional image