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LearnNB100-1405
antibody from Novus Biologicals
Targeting: USH1C
AIE-75, DFNB18, harmonin, NY-CO-37, NY-CO-38, PDZ-73, PDZ73, PDZD7C
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB100-1405 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1405, RRID:AB_2214138
- Product name
- Goat Polyclonal USH1C Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognize both reported isoforms (NP_005700.2 and NP_710142.1).
- Reactivity
- Human, Bovine
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Biochemical characterization of native Usher protein complexes from a vesicular subfraction of tracheal epithelial cells.
A defect in harmonin, a PDZ domain-containing protein expressed in the inner ear sensory hair cells, underlies Usher syndrome type 1C.
Zallocchi M, Sisson JH, Cosgrove D
Biochemistry 2010 Feb 16;49(6):1236-47
Biochemistry 2010 Feb 16;49(6):1236-47
A defect in harmonin, a PDZ domain-containing protein expressed in the inner ear sensory hair cells, underlies Usher syndrome type 1C.
Verpy E, Leibovici M, Zwaenepoel I, Liu XZ, Gal A, Salem N, Mansour A, Blanchard S, Kobayashi I, Keats BJ, Slim R, Petit C
Nature genetics 2000 Sep;26(1):51-5
Nature genetics 2000 Sep;26(1):51-5
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: USH1C Antibody [NB100-1405] - (0.1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: USH1C Antibody [NB100-1405] - (10ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
