LS-C114740

antibody from LSBio

Targeting: FCGR2A CD32, CD32A, CDw32, FCG2, FCGR2, FCGR2A1, IGFR2

Provider product page for LS-C114740

Western blot

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [0]
Comments [0]
Validations
Immunoprecipitation [3]
Immunohistochemistry [27]

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Product number: LS-C114740 - Provider product page
Provider: LSBio
Product name: CD32A Antibody (clone OTI9C6) LS-C114740
Antibody type: Monoclonal
Description: Protein A/G purified
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: OTI9C6
Storage: Store at -20°C. Avoid freeze-thaw cycles.

FCGR2A protein structure - LS-C114740 shown in red.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Immunoprecipitation(IP) of FCGR2A by using monoclonal anti-FCGR2A antibodies (Negative control: IP without adding anti-FCGR2A antibody.). For each experiment, 500ul of DDK tagged FCGR2A overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-FCGR2A antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Immunoprecipitation(IP) of FCGR2A by using monoclonal anti-FCGR2A antibodies (Negative control: IP without adding anti-FCGR2A antibody.). For each experiment, 500ul of DDK tagged FCGR2A overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-FCGR2A antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Immunoprecipitation(IP) of FCGR2A by using monoclonal anti-FCGR2A antibodies (Negative control: IP without adding anti-FCGR2A antibody.). For each experiment, 500ul of DDK tagged FCGR2A overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-FCGR2A antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: IHC of paraffin-embedded colon tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: IHC of paraffin-embedded Kidney tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: IHC of paraffin-embedded lung tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: IHC of paraffin-embedded Ovary tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: IHC of paraffin-embedded pancreas tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: IHC of paraffin-embedded thyroid tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: IHC of paraffin-embedded endometrium tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: IHC of paraffin-embedded breast tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: IHC of paraffin-embedded breast tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: IHC of paraffin-embedded colon tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: IHC of paraffin-embedded Kidney tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: IHC of paraffin-embedded lung tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: IHC of paraffin-embedded Ovary tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: IHC of paraffin-embedded pancreas tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: IHC of paraffin-embedded thyroid tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: IHC of paraffin-embedded endometrium tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: IHC of paraffin-embedded prostate tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: IHC of paraffin-embedded colon tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: IHC of paraffin-embedded Kidney tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: IHC of paraffin-embedded Ovary tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: IHC of paraffin-embedded pancreas tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: IHC of paraffin-embedded thyroid tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: IHC of paraffin-embedded endometrium tissue using anti-FCGR2A mouse monoclonal antibody. (Dilution 1:50).