OAAB05513

antibody from Aviva Systems Biology

Targeting: FCGR2A CD32, CD32A, CDw32, FCG2, FCGR2, FCGR2A1, IGFR2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

OAAB05513

Provider

Aviva Systems Biology

Product name

FCGR2A antibody - C-terminal region

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Vial size

400ul

Storage

Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Western blot analysis of FCGR2A (arrow) using rabbit polyclonal FCGR2A Antibody (C-term) either nontransfected (Lane 1) or lysates transiently transfected transfected (Lane 2) with the FCGR2A gene.

Sample type

lysatestransiently transfected with the FCGR2A gene.

Primary Ab dilution

1.0 µg/mL

Protocol

Protocol

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Western blot analysis of FCGR2A Antibody (C-term) in HL-60 cell lysates (35ug/lane). FCGR2A (arrow) was detected using the purified Pab.

Sample type

HL-60 cell line lysates

Primary Ab dilution

1.0 µg/mL

Protocol

Protocol

Flow cytometry

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

FCGR2A Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Sample type

MDA-MB435 cells

Primary Ab dilution

1.0 µg/mL

Protocol

Protocol