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LearnAM33398PU-N
antibody from Acris Antibodies GmbH
Targeting: FCGR2A
CD32, CD32A, CDw32, FCG2, FCGR2, FCGR2A1, IGFR2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- AM33398PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti CD32 / FcRII-a
- Antibody type
- Monoclonal
- Antigen
- Full length human recombinant protein of Human FCGR2A (NP_067674) produced in HEK293T cell.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 13D7
- Vial size
- 50 µl
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates(5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Anti-FCGR2A mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FCGR2A.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunoprecipitation(IP) of FCGR2A by using monoclonal anti-FCGR2A antibodies (Negativecontrol: IP without adding anti-FCGR2A antibody.). For each experiment, 500ul of DDKtagged FCGR2A overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-FCGR2A antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads weremixed and incubated overnight. After extensive wash to remove any non-specific binding, theimmuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
