60012FI

antibody from STEMCELL Technologies Inc

Targeting: FCGR2A CD32, CD32A, CDw32, FCG2, FCGR2, FCGR2A1, IGFR2

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

60012FI

Provider

STEMCELL Technologies Inc

Proper citation

STEMCELL Technologies Cat#60012FI, RRID:AB_2722545

Product name

Anti-Human CD32 Antibody, Clone IV.3, FITC

Antibody type

Monoclonal

Description

The IV.3 antibody reacts with human CD32 (Fc?RII), an ~40 kDa type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, immunomodulation and platelet aggregation. CD32 is encoded by three genes (A, B, C) and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. All isoforms are expressed by monocytes/macrophages, placental trophoblasts and endothelial cells. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. Isoform IIc is expressed by NK cells and neutrophils. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes. These interactions can result in non-specific labeling in antibody-based detection and cell separation experiments and the IV.3 antibody may be employed as a blocking antibody to reduce non-specific binding. The IV.3 antibody binds most strongly to the IIa isoforms of CD32, with the epitope mapped to amino acids 132 - 137 [FSHLDP] in domain 2, within the ligand binding site. Binding of the IV.3 antibody can be blocked by clone FLI8.26 in flow cytometry analyses, suggesting that these clones may share a common or overlapping epitope. This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Human T Cell Enrichment Kit (Catalog #19051) and EasySep™ Human CD4+ T Cell Enrichment Kit (Catalog #19052).

Reactivity

Human

Host

Human

Isotype

IgG

Vial size

100 Tests

Storage

2 - 8°C