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LearnCF500647
antibody from Invitrogen Antibodies
Targeting: FCGR2A
CD32, CD32A, CDw32, FCG2, FCGR2, FCGR2A1, IGFR2
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- CF500647 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FCGR2A Monoclonal Antibody (OTI19C10), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI19C10
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (RC205786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2. Positive lysates LY402871 (100 µg) and LC402871 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10 µg) from 1 cell line by using anti-FCGR2A monoclonal antibody at 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-FCGR2A mouse monoclonal antibody (TA500647) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FCGR2A(RC205786).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human tonsil within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA500647)(1:500).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC205786 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FCGR2A antibody (TA500647), and then analyzed by flow cytometry.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation(IP) of FCGR2A by using TrueMab monoclonal anti-FCGR2A antibodies (Negative control: IP without adding anti-FCGR2A antibody.). For each experiment, 500ul of tagged FCGR2A overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 µg of anti-FCGR2A antibody and 20ul (0.1mg) of goat anti-mouse conj µgated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
