303201

antibody from BioLegend

Targeting: FCGR2A CD32, CD32A, CDw32, FCG2, FCGR2, FCGR2A1, IGFR2

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

303201

Provider

BioLegend

Proper citation

BioLegend Cat#303201, RRID:AB_314333

Product name

Purified anti-human CD32

Antibody type

Monoclonal

Antigen

CD32

Description

CD32 is a 40 kD polymorphic transmembrane glycoprotein also known as FcγRII and FCRII. It is an immunoglobulin superfamily member expressed on monocytes/macrophages, granulocytes, platelets and B cells. There are at least 6 isoforms of CD32 resulting from alternative mRNA splicing. CD32 mediates phagocytosis and oxidative burst in granulocytes, as well as platelet aggregation and immunomodulation. The extracellular domain of CD32 binds to polymeric and aggregated IgG and immune complexes, while the intracellular domain has been reported to associate with SHP-1 (B1 isoform).

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

25 µg

Concentration

0.5 mg/ml

Storage

The antibody solution should be stored undiluted at 4°C.

References

Submitted references

Toll-like receptor 2 mediates the activation of human monocytes and endothelial cells by antiphospholipid antibodies.

Satta N, Kruithof EK, Fickentscher C, Dunoyer-Geindre S, Boehlen F, Reber G, Burger D, de Moerloose P
Blood 2011 May 19;117(20):5523-31

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C-reactive protein and annexin A5 bind to distinct sites of negatively charged phospholipids present in oxidized low-density lipoprotein.

van Tits L, de Graaf J, Toenhake H, van Heerde W, Stalenhoef A
Arteriosclerosis, thrombosis, and vascular biology 2005 Apr;25(4):717-22

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood lymphocytes stained with purified FUN-2, followed by anti-mouse IgGs FITC

Conjugate

Unconjugated