Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF935 - Provider product page
- Provider
- R&D Systems
- Product name
- Human ADAM15 Ectodomain Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human ADAM15 Ectodomain in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse (rm) ADAM15 is observed, and less than 1% cross-reactivity with rmADAM9, rmADAM10, recombinant human (rh) ADAM8, rhADAM9, rhADAM10, rhADAM12, rhADAM17 (TACE), rhADAM19, rhADAM22, rhADAM23, rhADAM32, and rhADAM33 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q13444- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Alternative splicing of ADAM15 regulates its interactions with cellular SH3 proteins.
ADAM15 upregulation and interaction with multiple binding partners in inflammatory bowel disease.
Kleino I, Ortiz RM, Yritys M, Huovila AP, Saksela K
Journal of cellular biochemistry 2009 Nov 1;108(4):877-85
Journal of cellular biochemistry 2009 Nov 1;108(4):877-85
ADAM15 upregulation and interaction with multiple binding partners in inflammatory bowel disease.
Mosnier JF, Jarry A, Bou-Hanna C, Denis MG, Merlin D, Laboisse CL
Laboratory investigation; a journal of technical methods and pathology 2006 Oct;86(10):1064-73
Laboratory investigation; a journal of technical methods and pathology 2006 Oct;86(10):1064-73
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- ADAM15 in Human Prostate. ADAM15 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human ADAM15 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF935) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
