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LearnPA5-21611
antibody from Invitrogen Antibodies
Targeting: NR2F1
COUP-TFI, COUPTF1, EAR-3, ERBAL3, SVP44, TCFCOUP1, TFCOUP1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-21611 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NR2F1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: COUP TF1 tansfected 293T cell. Predicted reactivity: Mouse (100%), Rat (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references G-quadruplex DNA structures in human stem cells and differentiation.
Zyner KG, Simeone A, Flynn SM, Doyle C, Marsico G, Adhikari S, Portella G, Tannahill D, Balasubramanian S
Nature communications 2022 Jan 10;13(1):142
Nature communications 2022 Jan 10;13(1):142
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of COUP-TFI in non-transfected lysate 293T lysate (A) and transfected lysate (B). Samples were resolved on 12% SDS PAGE gel and probed using a COUP-TFI polyclonal antibody (Product # PA5-21611) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NR2F1 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a NR2F1 Polyclonal Antibody (Product # PA5-21611) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NR2F1 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a NR2F1 Polyclonal Antibody (Product # PA5-21611) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of COUP-TFI in paraformaldehyde-fixed HeLa cells using a COUP-TFI polyclonal antibody (Product # PA5-21611) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using COUP TF1 (Product # PA5-21611) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NR2F1 Polyclonal Antibody detects COUP TF1 protein at nucleus on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. NR2F1 Polyclonal Antibody (Product # PA5-21611) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
