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LearnNBP1-31259
antibody from Novus Biologicals
Targeting: NR2F1
COUP-TFI, COUPTF1, EAR-3, ERBAL3, SVP44, TCFCOUP1, TFCOUP1
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP1-31259 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-31259, RRID:AB_2155486
- Product name
- Rabbit Polyclonal COUP-TF I/NR2F1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Cell stress in cortical organoids impairs molecular subtype specification.
Bhaduri A, Andrews MG, Mancia Leon W, Jung D, Shin D, Allen D, Jung D, Schmunk G, Haeussler M, Salma J, Pollen AA, Nowakowski TJ, Kriegstein AR
Nature 2020 Feb;578(7793):142-148
Nature 2020 Feb;578(7793):142-148
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: COUP-TF I/NR2F1 Antibody [NBP1-31259] - Analysis of NR2F1 expression in transfected 293T cell line by NR2F1 polyclonal antibody. A: Non-transfected lysate. B: NR2F1 transfected lysate diluted at 1:500
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: COUP-TF I/NR2F1 Antibody [NBP1-31259] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with COUP TF1 antibody [N1], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: COUP-TF I/NR2F1 Antibody [NBP1-31259] - Staining shows detection of the protein at nucleus on a Paraffin-embedded mouse hind brain sample.
