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antibody from Invitrogen Antibodies
Targeting: NR2F1
COUP-TFI, COUPTF1, EAR-3, ERBAL3, SVP44, TCFCOUP1, TFCOUP1
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Chromatin Immunoprecipitation [1]
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- Product number
- PA5-30190 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NR2F1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, HCT116. Predicted reactivity: Mouse (100%), Rat (99%), Xenopus laevis (98%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.92 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NR2F1 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a NR2F1 Polyclonal Antibody (Product # PA5-30190) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NR2F1 Polyclonal Antibody (Product # PA5-30190). Sample (30 µg of whole cell lysate). Lane A: HCT116. 10% SDS PAGE. NR2F1 Polyclonal Antibody (Product # PA5-30190) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation (ChIP) assay of endogenous NR2F1 protein using Anti-NR2F1 Antibody: ChIP was performed using Anti-NR2F1 Polyclonal Antibody (Product # PA5-30190, 2.5 µg) on sheared chromatin from MCF7 cells using the MAGnify ChIP System kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR using primers binding to DLX1 transcription start site, EGR1 exon 2, SNAI1 promoter and SAT2 satellite repeats. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
