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- Antibody Data
- Antigen structure
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- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AM33441PU-N - Provider product page
- Provider
- OriGene
- Product name
- ARMETL1 (CDNF) mouse monoclonal antibody, clone 7G5, Aff - Purified
- Antibody type
- Monoclonal
- Description
- ARMETL1 (CDNF) mouse monoclonal antibody, clone 7G5, Aff - Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CDNF
- Isotype
- IgG
- Antibody clone number
- 7G5
- Vial size
- 100 µg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot testing of anti-CDNF monoclonal antibody (6G5). Line 1:? PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Line 2:? Recombinant CDNF expressed into the supernatant of CHO cell culture medium.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence detection of human CDNF expressed in U2OS cells. CDNF was visualized using anti-CDNF antibody clone 6G5 at 1 ug/ml. Goat ant-mouse AlexaFluor488 was used as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for CDNF specific signal and blue for nuclei. A. CDNF-expressing U2OS cells; B. Negative control (non-transfected U2OS cells).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry testing of anti-CDNF monoclonal antibody 6G5. Analysis was performed using formalin-fixed paraffin-embedded human cerebral cortex tissue sections from Alzheimers disease patients. Tissue sections were boiled with sodium citrate buffer (pH 6) for antigen retrieval. Incubation with primary antibody at 5 ug/ml was performed overnight at 4°C. DAKO EnVisionTM Detection System, Peroxidase/DAB was used for visualization. Sections were counterstained with toluidine blue and mounted with Eukitt mounting medium. A. CDNF staining by monoclonal antibody 6G5; B. Negative staining without primary antibody.
- Validation comment
- IHC
