21021-1-AP

antibody from Invitrogen Antibodies

Targeting: PDXDC1 KIAA0251

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

21021-1-AP

Provider

Invitrogen Antibodies

Product name

PDXDC1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MDASLEKIA DPTLAEMGKN LKEAVKMLED SQRRTEEENG KKLISGDIPG PLQGSGQDMV SILQLVQNLM HGDEDEEPQS PRIQNIGEQG HMALLGHSLG AYISTLDKEK LRKLTTRILS DTTLWLCRIF RYENGCAYFH EEEREGLAKI CRLAIHSRYE DFVVDGFNVL YNKKPVIYLS AAARPGLGQY LCNQDVAFLE KLIKDDIERG RLPLLLVANA GTAAVGHTDK IGRLKELCEQ YGIWLHVEGV NLATLALGYV SSSVLAAAKC DSMTMTPGPW LGLPAVPAVT LYKHDDPALT LVAGLTSNKP TDKLRALPLW LSLQYLGLDG FVERIKHACQ LSQRLQ (1-345 aa encoded by B C033748)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.39 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 21021-1-AP (PDXDC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 21021-1-AP (PDXDC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using PDXDC1 antibody 21021-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using PDXDC1 antibody 21021-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis using 21021-1-AP (PDXDC1 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis using 21021-1-AP (PDXDC1 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human hepatocirrhosis using 21021-1-AP (PDXDC1 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human hepatocirrhosis using 21021-1-AP (PDXDC1 antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PDXDC1 (IP:21021-1-AP, 4 ug; Detection:21021-1-AP 1:500) with HepG2 cells lysate 1680 ug.