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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB3157 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Wnt-9a Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Wnt-9a in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Wnt-2, -3a, -6, -7a, -7b, recombinant mouse Wnt-1, -2b, -4, -5a, -5b, -8a, -8b, -9b, -10a, -10b, -11, or -16 is observed.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Wnt-9a by Western Blot. Western blot shows lysates of KATO-III human gastric carcinoma cell line and ME-180 human cervical epithelial carcinoma cell line. PVDF membrane was probed with 5 µg/mL Mouse Anti-Human Wnt-9a Monoclonal Antibody (Catalog # MAB3157) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human Wnt-9a (20 ng) was included. Specific bands for native Wnt-9a were detected at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Wnt-9a in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human Wnt-9a Monoclonal Antibody (Catalog # MAB3157, filled histogram) or isotype control AA175antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
