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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Flow cytometry [2]
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Validation data
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- Product number
- MA5-24050 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WNT9A Monoclonal Antibody (326711)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, no cross-reactivity with recombinant human Wnt-2, -3a, -6, -7a, -7b, recombinant mouse Wnt-1, -2b, -4, -5a, -5b, -8a, -8b, -9b, -10a, -10b, -11, or -16 is observed.
- Antibody clone number
- 326711
- Concentration
- 0.5 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of KATO-III human gastric carcinoma cell line and ME-180 human cervical epithelial carcinoma cell line. PVDF membrane was probed with 5 µg/mL mouse Anti-human Wnt-9a Monoclonal Antibody (Product # MA5-24050) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. For additional reference, recombinant human Wnt-9a (20 ng) was included. Specific bands for native Wnt-9a were detected at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of WNT9A in KATO-III human gastric carcinoma cell line and ME‚180 human cervical epithelial carcinoma cell line. Samples were incubated in WNT9A monoclonal antibody (Product # MA5-24050) using a dilution of 5 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. Specific bands for native Wnt-9a were detected at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MCF-7 human breast cancer cell line was stained with mouse Anti-human Wnt-9a Monoclonal Antibody (Product # MA5-24050) or isotype control AA175antibodyopen histogram), followed by Allophycocyanin-conjugated Anti-mouse IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of WNT9A in MCF-7 human breast cancer cell line. Samples were incubated in WNT9A monoclonal antibody (Product # MA5-24050) or isotype control AA175antibody followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
