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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- AF3636 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CMG-2/ANTXR2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse CMG-2/ANTXR2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human CMG-2 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q6DFX2- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 1;183(9):5799-806
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 1;183(9):5799-806
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Mouse CMG-2/ANTXR2 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line, mouse thymus tissue, and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Mouse CMG-2/ANTXR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3636) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CMG-2/ANTXR2 at approximately 55-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
