PA5-67257

antibody from Invitrogen Antibodies

Targeting: KIAA0319L AAVR, KIAA1837

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-67257

Provider

Invitrogen Antibodies

Product name

KIAA0319L Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: KRKSKYKILDA TDQESLELKP TSRAGIKQKG LLLSSSLMHS ESELDSDDAI FTWPDREKGK LLHGQNGSVP NGQ

Concentration

0.25 mg/mL

References

Submitted references

Identification of AAV serotypes for lung gene therapy in human embryonic stem cell-derived lung organoids.

Meyer-Berg H, Zhou Yang L, Pilar de Lucas M, Zambrano A, Hyde SC, Gill DR
Stem cell research & therapy 2020 Oct 23;11(1):448

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of KIAA0319L in human cell line U-2 OS shows localization to the Golgi apparatus. Samples were probed using a KIAA0319L Polyclonal Antibody (Product # PA5-67257).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 3 Immunohistochemistry for markers of alveolar type II cells and AAV entry receptors. Representative images are shown of fixed-frozen sections of LBOs ( n = 2-3), with nuclei stained with DAPI (blue) and various markers (red), including ATII cell marker SP-C ( a ), ATII cell marker SP-B ( b , c ), universal AAV co-receptor AAVR ( d ), glycan receptor alpha-2,3-linked sialic acid ( e , f ) and glycan receptor heparan sulphate ( g , h ). Native fluorescence is observed following microinjection of AAV6.2-CMV-eGFP ( c ) compared with negative control LBOs ( b ). Sections digested with sialidase A ( e ) and heparinase III ( g ) to remove glycans are included as negative staining controls. Staining controls for images a - d are shown in suppl. Fig. 2 . Scale bar 50 mum