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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 691602 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#691602, RRID:AB_2632653
- Product name
- Purified anti-Artemis
- Antibody type
- Monoclonal
- Description
- Artemis, also known as DCLRE1C, is a nuclear protein belonging to the metallo-β-lactamase superfamily. Artemis has been found to be required for the assembling of B cell antibody genes and T cell receptor genes from individual V, D, and J segments. During V(D)J recombination, Artemis forms a complex with DNA-dependent protein kinase (DNAâPK) and cuts the DNA with hairpin structure. Deficiency in Artemis results in blocking of V(D)J recombination and failure to produce mature B and T cells, which is known as the condition of severe combined immune deficiency (SCID). Artemis is also found to be involved in the termini processing step of double-strand break (DSB) repair. Artemis-deficient cells are more sensitive to DSB induced by ionizing radiation or chemical agents such as bleomycin and neocarzinostatin.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 15E7C90
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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- BioLegend (provider)
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- Experimental details
- Total cell lysate from human HeLa cells (lane 1) and mouse RAW264.7 cells (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-Artemis (clone 15E7C90) antibody. Proteins were visualized using an HRP goat anti-mouse IgG antibody and chemiluminescence detection. Purified anti-β-actin (clone Poly6221) antibody was used as a loading control.
- Conjugate
- Unconjugated
