PAB20855

antibody from Abnova Corporation

Targeting: CISD2 ERIS, Miner1, NAF-1, WFS2, ZCD2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20855

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20855, RRID:AB_10965633

Product name

CISD2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant CISD2.

Antigen sequence

PKKKQQKDSLINLKIQKENPKVVNEINIEDLCLTK
AAYCRCWRSKTFPACDGSHNKHNELTGDNVGPLIL
KKKEV


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with CISD2 polyclonal antibody (Cat # PAB20855) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with CISD2 polyclonal antibody (Cat # PAB20855) at 1-4 ug/mL dilution shows positivity in endoplasmic reticulum.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas with CISD2 polyclonal antibody (Cat # PAB20855) shows strong cytoplasmic positivity in exocrine glandular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)