Antibody data
- Antibody Data
- Antigen structure
- References [16]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- 55054-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#55054-1-AP, RRID:AB_10792932
- Product name
- USP9X antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated USP9X antibody (Cat. #55054-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references A Proteomic Approach for Systematic Mapping of Substrates of Human Deubiquitinating Enzymes.
USP9X-mediated KDM4C deubiquitination promotes lung cancer radioresistance by epigenetically inducing TGF-β2 transcription.
Integrative oncogene-dependency mapping identifies RIT1 vulnerabilities and synergies in lung cancer.
Antagonistic activities of CDC14B and CDK1 on USP9X regulate WT1-dependent mitotic transcription and survival.
Arginine methylation-dependent LSD1 stability promotes invasion and metastasis of breast cancer.
Inhibition of USP9X Downregulates JAK2-V617F and Induces Apoptosis Synergistically with BH3 Mimetics Preferentially in Ruxolitinib-Persistent JAK2-V617F-Positive Leukemic Cells.
Detailed Dissection of UBE3A-Mediated DDI1 Ubiquitination.
Inhibition of the deubiquitinase USP9x induces pre-B cell homeobox 1 (PBX1) degradation and thereby stimulates prostate cancer cell apoptosis.
Deubiquitinase USP9X regulates the invasion of prostate cancer cells by regulating the ERK pathway and mitochondrial dynamics.
USP9X-mediated deubiquitination of B-cell CLL/lymphoma 9 potentiates Wnt signaling and promotes breast carcinogenesis.
The deubiquitinase USP9X promotes tumor cell survival and confers chemoresistance through YAP1 stabilization.
Deubiquitinating enzyme USP9X regulates cellular clock function by modulating the ubiquitination and degradation of a core circadian protein BMAL1.
USP9X Limits Mitotic Checkpoint Complex Turnover to Strengthen the Spindle Assembly Checkpoint and Guard against Chromosomal Instability.
Deubiquitinating Enzyme USP9X Suppresses Tumor Growth via LATS Kinase and Core Components of the Hippo Pathway.
USP9X regulates centrosome duplication and promotes breast carcinogenesis.
The X-linked deubiquitinase USP9X is an integral component of centrosome.
Ramirez J, Prieto G, Olazabal-Herrero A, Borràs E, Fernandez-Vigo E, Alduntzin U, Osinalde N, Beaskoetxea J, Lectez B, Aloria K, Rodriguez JA, Paradela A, Sabidó E, Muñoz J, Corrales F, Arizmendi JM, Mayor U
International journal of molecular sciences 2021 May 3;22(9)
International journal of molecular sciences 2021 May 3;22(9)
USP9X-mediated KDM4C deubiquitination promotes lung cancer radioresistance by epigenetically inducing TGF-β2 transcription.
Jie X, Fong WP, Zhou R, Zhao Y, Zhao Y, Meng R, Zhang S, Dong X, Zhang T, Yang K, Wu G, Xu S
Cell death and differentiation 2021 Jul;28(7):2095-2111
Cell death and differentiation 2021 Jul;28(7):2095-2111
Integrative oncogene-dependency mapping identifies RIT1 vulnerabilities and synergies in lung cancer.
Vichas A, Riley AK, Nkinsi NT, Kamlapurkar S, Parrish PCR, Lo A, Duke F, Chen J, Fung I, Watson J, Rees M, Gabel AM, Thomas JD, Bradley RK, Lee JK, Hatch EM, Baine MK, Rekhtman N, Ladanyi M, Piccioni F, Berger AH
Nature communications 2021 Aug 9;12(1):4789
Nature communications 2021 Aug 9;12(1):4789
Antagonistic activities of CDC14B and CDK1 on USP9X regulate WT1-dependent mitotic transcription and survival.
Dietachmayr M, Rathakrishnan A, Karpiuk O, von Zweydorf F, Engleitner T, Fernández-Sáiz V, Schenk P, Ueffing M, Rad R, Eilers M, Gloeckner CJ, Clemm von Hohenberg K, Bassermann F
Nature communications 2020 Mar 9;11(1):1268
Nature communications 2020 Mar 9;11(1):1268
Arginine methylation-dependent LSD1 stability promotes invasion and metastasis of breast cancer.
Liu J, Feng J, Li L, Lin L, Ji J, Lin C, Liu L, Zhang N, Duan D, Li Z, Huang B, Zhang Y, Lu J
EMBO reports 2020 Feb 5;21(2):e48597
EMBO reports 2020 Feb 5;21(2):e48597
Inhibition of USP9X Downregulates JAK2-V617F and Induces Apoptosis Synergistically with BH3 Mimetics Preferentially in Ruxolitinib-Persistent JAK2-V617F-Positive Leukemic Cells.
Akiyama H, Umezawa Y, Watanabe D, Okada K, Ishida S, Nogami A, Miura O
Cancers 2020 Feb 10;12(2)
Cancers 2020 Feb 10;12(2)
Detailed Dissection of UBE3A-Mediated DDI1 Ubiquitination.
Elu N, Osinalde N, Beaskoetxea J, Ramirez J, Lectez B, Aloria K, Rodriguez JA, Arizmendi JM, Mayor U
Frontiers in physiology 2019;10:534
Frontiers in physiology 2019;10:534
Inhibition of the deubiquitinase USP9x induces pre-B cell homeobox 1 (PBX1) degradation and thereby stimulates prostate cancer cell apoptosis.
Liu Y, Xu X, Lin P, He Y, Zhang Y, Cao B, Zhang Z, Sethi G, Liu J, Zhou X, Mao X
The Journal of biological chemistry 2019 Mar 22;294(12):4572-4582
The Journal of biological chemistry 2019 Mar 22;294(12):4572-4582
Deubiquitinase USP9X regulates the invasion of prostate cancer cells by regulating the ERK pathway and mitochondrial dynamics.
Zhang J, Wang J, Luan T, Zuo Y, Chen J, Zhang H, Ye Z, Wang H, Hai B
Oncology reports 2019 Jun;41(6):3292-3304
Oncology reports 2019 Jun;41(6):3292-3304
USP9X-mediated deubiquitination of B-cell CLL/lymphoma 9 potentiates Wnt signaling and promotes breast carcinogenesis.
Shang Z, Zhao J, Zhang Q, Cao C, Tian S, Zhang K, Liu L, Shi L, Yu N, Yang S
The Journal of biological chemistry 2019 Jun 21;294(25):9844-9857
The Journal of biological chemistry 2019 Jun 21;294(25):9844-9857
The deubiquitinase USP9X promotes tumor cell survival and confers chemoresistance through YAP1 stabilization.
Li L, Liu T, Li Y, Wu C, Luo K, Yin Y, Chen Y, Nowsheen S, Wu J, Lou Z, Yuan J
Oncogene 2018 May;37(18):2422-2431
Oncogene 2018 May;37(18):2422-2431
Deubiquitinating enzyme USP9X regulates cellular clock function by modulating the ubiquitination and degradation of a core circadian protein BMAL1.
Zhang Y, Duan C, Yang J, Chen S, Liu Q, Zhou L, Huang Z, Xu Y, Xu G
The Biochemical journal 2018 Apr 30;475(8):1507-1522
The Biochemical journal 2018 Apr 30;475(8):1507-1522
USP9X Limits Mitotic Checkpoint Complex Turnover to Strengthen the Spindle Assembly Checkpoint and Guard against Chromosomal Instability.
Skowyra A, Allan LA, Saurin AT, Clarke PR
Cell reports 2018 Apr 17;23(3):852-865
Cell reports 2018 Apr 17;23(3):852-865
Deubiquitinating Enzyme USP9X Suppresses Tumor Growth via LATS Kinase and Core Components of the Hippo Pathway.
Toloczko A, Guo F, Yuen HF, Wen Q, Wood SA, Ong YS, Chan PY, Shaik AA, Gunaratne J, Dunne MJ, Hong W, Chan SW
Cancer research 2017 Sep 15;77(18):4921-4933
Cancer research 2017 Sep 15;77(18):4921-4933
USP9X regulates centrosome duplication and promotes breast carcinogenesis.
Li X, Song N, Liu L, Liu X, Ding X, Song X, Yang S, Shan L, Zhou X, Su D, Wang Y, Zhang Q, Cao C, Ma S, Yu N, Yang F, Wang Y, Yao Z, Shang Y, Shi L
Nature communications 2017 Mar 31;8:14866
Nature communications 2017 Mar 31;8:14866
The X-linked deubiquitinase USP9X is an integral component of centrosome.
Wang Q, Tang Y, Xu Y, Xu S, Jiang Y, Dong Q, Zhou Y, Ge W
The Journal of biological chemistry 2017 Aug 4;292(31):12874-12884
The Journal of biological chemistry 2017 Aug 4;292(31):12874-12884
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 55054-1-AP(USP9X antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The USP9X antibody from Proteintech is a rabbit polyclonal antibody to a peptide of human USP9X. This antibody recognizes human,mouse,rat antigen. The USP9X antibody has been validated for the following applications: ELISA, WB, IF, IHC analysis.