Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ARP59340_P050 - Provider product page
- Provider
- Aviva Systems Biology
- Proper citation
- Aviva Systems Biology Cat#ARP59340_P050, RRID:AB_10883010
- Product name
- USP9X antibody - C-terminal region (ARP59340_P050)
- Antibody type
- Polyclonal
- Antigen
- The immunogen is a synthetic peptide directed towards the C terminal region of human USP9X
- Description
- Affinity Purified
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Horse, Porcine, Rabbit
- Host
- Rabbit
- Vial size
- 100 μl
- Concentration
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Storage
- For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Sample Type: 1. total HeLa cell extract (100µg)2. total HeLa cell extract incubated with HA-UbVME (100µg)3. Rat Liver cytosolic extract (100µg)4. Rat Liver cytosolic extract incubated with HA-UbVME (100µg)Primary Dilution: 1:1000 Secondary Antibody: alkaline phosphatase-conjugated anti-rabbit Secondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, Portugal
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Immunohistochemistry with SRC transformed 3T3 cells using Anti-USP9X antibody
- Sample type
- SRC transformed 3T3 cells
- Validation comment
- The subcellular location is supported by literature.
- Primary Ab dilution
- 1/50