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ELISAAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00008239-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00008239-M01, RRID:AB_509098
- Product name
- USP9X monoclonal antibody (M01), clone 1C4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant USP9X.
- Antigen sequence
MTATTRGSPVGGNDNQGQAPDGQSQPPLQQNQTSS
PDSSNENSPATPPDEQGQGDAPPQLEDEEPAFPHT
DLAKLDDMINRPRWVVPVLP- Isotype
- IgG
- Antibody clone number
- 1C4
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Role of Angiomotin-like 2 mono-ubiquitination on YAP inhibition.
Deubiquitylating enzyme USP9x regulates radiosensitivity in glioblastoma cells by Mcl-1-dependent and -independent mechanisms.
Role of Ku70 in deubiquitination of Mcl-1 and suppression of apoptosis.
Deubiquitinase USP9x confers radioresistance through stabilization of Mcl-1.
Mcl-1 Phosphorylation defines ABT-737 resistance that can be overcome by increased NOXA expression in leukemic B cells.
Kim M, Kim M, Park SJ, Lee C, Lim DS
EMBO reports 2016 Jan;17(1):64-78
EMBO reports 2016 Jan;17(1):64-78
Deubiquitylating enzyme USP9x regulates radiosensitivity in glioblastoma cells by Mcl-1-dependent and -independent mechanisms.
Wolfsperger F, Hogh-Binder SA, Schittenhelm J, Psaras T, Ritter V, Bornes L, Huber SM, Jendrossek V, Rudner J
Cell death & disease 2016 Jan 14;7(1):e2039
Cell death & disease 2016 Jan 14;7(1):e2039
Role of Ku70 in deubiquitination of Mcl-1 and suppression of apoptosis.
Wang B, Xie M, Li R, Owonikoko TK, Ramalingam SS, Khuri FR, Curran WJ, Wang Y, Deng X
Cell death and differentiation 2014 Jul;21(7):1160-9
Cell death and differentiation 2014 Jul;21(7):1160-9
Deubiquitinase USP9x confers radioresistance through stabilization of Mcl-1.
Trivigno D, Essmann F, Huber SM, Rudner J
Neoplasia (New York, N.Y.) 2012 Oct;14(10):893-904
Neoplasia (New York, N.Y.) 2012 Oct;14(10):893-904
Mcl-1 Phosphorylation defines ABT-737 resistance that can be overcome by increased NOXA expression in leukemic B cells.
Mazumder S, Choudhary GS, Al-Harbi S, Almasan A
Cancer research 2012 Jun 15;72(12):3069-79
Cancer research 2012 Jun 15;72(12):3069-79
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged USP9X is approximately 0.1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to USP9X on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
