Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00008239-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00008239-M01, RRID:AB_549092
- Product name
- Mouse Monoclonal USP9x Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. USP9X - ubiquitin specific peptidase 9, X-linked (fat facets-like, Drosophila)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Role of Angiomotin-like 2 mono-ubiquitination on YAP inhibition.
Deubiquitylating enzyme USP9x regulates radiosensitivity in glioblastoma cells by Mcl-1-dependent and -independent mechanisms.
Role of Ku70 in deubiquitination of Mcl-1 and suppression of apoptosis.
Deubiquitinase USP9x confers radioresistance through stabilization of Mcl-1.
Deubiquitinase USP9x confers radioresistance through stabilization of Mcl-1.
Mcl-1 Phosphorylation defines ABT-737 resistance that can be overcome by increased NOXA expression in leukemic B cells.
The Bcl-xL inhibitor, ABT-737, efficiently induces apoptosis and suppresses growth of hepatoma cells in combination with sorafenib.
Deubiquitinase USP9X stabilizes MCL1 and promotes tumour cell survival.
Kim M, Kim M, Park SJ, Lee C, Lim DS
EMBO reports 2016 Jan;17(1):64-78
EMBO reports 2016 Jan;17(1):64-78
Deubiquitylating enzyme USP9x regulates radiosensitivity in glioblastoma cells by Mcl-1-dependent and -independent mechanisms.
Wolfsperger F, Hogh-Binder SA, Schittenhelm J, Psaras T, Ritter V, Bornes L, Huber SM, Jendrossek V, Rudner J
Cell death & disease 2016 Jan 14;7(1):e2039
Cell death & disease 2016 Jan 14;7(1):e2039
Role of Ku70 in deubiquitination of Mcl-1 and suppression of apoptosis.
Wang B, Xie M, Li R, Owonikoko TK, Ramalingam SS, Khuri FR, Curran WJ, Wang Y, Deng X
Cell death and differentiation 2014 Jul;21(7):1160-9
Cell death and differentiation 2014 Jul;21(7):1160-9
Deubiquitinase USP9x confers radioresistance through stabilization of Mcl-1.
Trivigno D, Essmann F, Huber SM, Rudner J
Neoplasia (New York, N.Y.) 2012 Oct;14(10):893-904
Neoplasia (New York, N.Y.) 2012 Oct;14(10):893-904
Deubiquitinase USP9x confers radioresistance through stabilization of Mcl-1.
Trivigno D, Essmann F, Huber SM, Rudner J
Neoplasia (New York, N.Y.) 2012 Oct;14(10):893-904
Neoplasia (New York, N.Y.) 2012 Oct;14(10):893-904
Mcl-1 Phosphorylation defines ABT-737 resistance that can be overcome by increased NOXA expression in leukemic B cells.
Mazumder S, Choudhary GS, Al-Harbi S, Almasan A
Cancer research 2012 Jun 15;72(12):3069-79
Cancer research 2012 Jun 15;72(12):3069-79
The Bcl-xL inhibitor, ABT-737, efficiently induces apoptosis and suppresses growth of hepatoma cells in combination with sorafenib.
Hikita H, Takehara T, Shimizu S, Kodama T, Shigekawa M, Iwase K, Hosui A, Miyagi T, Tatsumi T, Ishida H, Li W, Kanto T, Hiramatsu N, Hayashi N
Hepatology (Baltimore, Md.) 2010 Oct;52(4):1310-21
Hepatology (Baltimore, Md.) 2010 Oct;52(4):1310-21
Deubiquitinase USP9X stabilizes MCL1 and promotes tumour cell survival.
Schwickart M, Huang X, Lill JR, Liu J, Ferrando R, French DM, Maecker H, O'Rourke K, Bazan F, Eastham-Anderson J, Yue P, Dornan D, Huang DC, Dixit VM
Nature 2010 Jan 7;463(7277):103-7
Nature 2010 Jan 7;463(7277):103-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: USP9x Antibody (1C4) [H00008239-M01] - USP9X expression in Hela NE ( Cat # L013V3 ).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: USP9x Antibody (1C4) [H00008239-M01] - USP9x in mouse ovarian cancer cell lysate using anti-USP9x antibody. The primary antibody was used at a dilution of 1:500 and incubated for overnight at 4C in 5% milk with PBST. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: USP9x Antibody (1C4) [H00008239-M01] - Detection limit for recombinant GST tagged USP9X is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: USP9x Antibody (1C4) [H00008239-M01] - Analysis of monoclonal antibody to USP9X on HeLa cell. Antibody concentration 10 ug/ml.