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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- MA5-34731 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- USP9X Recombinant Rabbit Monoclonal Antibody (JG35-11)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: SiHa, A549, 293, mouse colon tissue, 293T, F9, human tonsil tissue, mouse fallopian tube tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JG35-11
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of USP9x in Lane 1: SiHa, Lane 2: A549, Lane 3: 293, Lane 4: Mouse colon. Samples were incubated with USP9x monoclonal antibody (Product # MA5-34731), at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of USP9x in SiHa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with USP9x monoclonal antibody (Product # MA5-34731), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of USP9x in F9 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with USP9x monoclonal antibody (Product # MA5-34731), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of USP9x in 293T cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with USP9x monoclonal antibody (Product # MA5-34731), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of USP9x in paraffin-embedded mouse fallopian tube tissue. Samples were heat mediated antigen retrieval with sodium citrate buffer (pH6, 20 min), incubated with USP9x monoclonal antibody (Product # MA5-34731), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of USP9x in paraffin-embedded human tonsil tissue. Samples were heat mediated antigen retrieval with sodium citrate buffer (pH6, 20 min), incubated with USP9x monoclonal antibody (Product # MA5-34731), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of USP9x in 293T cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with USP9x monoclonal antibody (Product # MA5-34731) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
