NB100-2214

antibody from Novus Biologicals

Targeting: JARID2 JMJ

Provider product page for NB100-2214

Western blot

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Chromatin Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [17]
Comments [0]
Validations
Western blot [3]
Immunohistochemistry [1]

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Product number: NB100-2214 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#NB100-2214, RRID:AB_10000529
Product name: Rabbit Polyclonal Jumonji/JARID2 Antibody
Antibody type: Polyclonal
Description: Immunogen affinity purified.
Reactivity: Human, Mouse, Rabbit
Host: Rabbit
Isotype: IgG
Vial size: 0.1 ml
Concentration: 1.03 mg/ml
Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

JARID2 protein structure - NB100-2214 shown in red.

Submitted references PHF19 mediated regulation of proliferation and invasiveness in prostate cancer cells.

Jain P, Ballare C, Blanco E, Vizan P, Di Croce L
eLife 2020 Mar 10;9

Activation-Induced Cytidine Deaminase Regulates Fibroblast Growth Factor/Extracellular Signal-Regulated Kinases Signaling to Achieve the Naïve Pluripotent State During Reprogramming.

Kumar R, Evans T
Stem cells (Dayton, Ohio) 2019 Aug;37(8):1003-1017

Dlk1-Dio3 locus-derived lncRNAs perpetuate postmitotic motor neuron cell fate and subtype identity.

Yen YP, Hsieh WF, Tsai YY, Lu YL, Liau ES, Hsu HC, Chen YC, Liu TC, Chang M, Li J, Lin SP, Hung JH, Chen JA
eLife 2018 Oct 12;7

MTF2 recruits Polycomb Repressive Complex 2 by helical-shape-selective DNA binding.

Perino M, van Mierlo G, Karemaker ID, van Genesen S, Vermeulen M, Marks H, van Heeringen SJ, Veenstra GJC
Nature genetics 2018 Jul;50(7):1002-1010

Micro-ribonucleic acid-155 is a direct target of Meis1, but not a driver in acute myeloid leukemia.

Schneider E, Staffas A, Röhner L, Malmberg ED, Ashouri A, Krowiorz K, Pochert N, Miller C, Wei SY, Arabanian L, Buske C, Döhner H, Bullinger L, Fogelstrand L, Heuser M, Döhner K, Xiang P, Ruschmann J, Petriv OI, Heravi-Moussavi A, Hansen CL, Hirst M, Humphries RK, Rouhi A, Palmqvist L, Kuchenbauer F
Haematologica 2018 Feb;103(2):246-255

c-Myc is Required for BRAF(V600E)-Induced Epigenetic Silencing by H3K27me3 in Tumorigenesis.

Qu Y, Yang Q, Liu J, Shi B, Ji M, Li G, Hou P
Theranostics 2017;7(7):2092-2107

Epigenetic mechanisms underlying maternal diabetes-associated risk of congenital heart disease.

Basu M, Zhu JY, LaHaye S, Majumdar U, Jiao K, Han Z, Garg V
JCI insight 2017 Oct 19;2(20)

Chromatin association of XRCC5/6 in the absence of DNA damage depends on the XPE gene product DDB2.

Fantini D, Huang S, Asara JM, Bagchi S, Raychaudhuri P
Molecular biology of the cell 2017 Jan 1;28(1):192-200

Differential Sensitivity of Target Genes to Translational Repression by miR-17~92.

Jin HY, Oda H, Chen P, Yang C, Zhou X, Kang SG, Valentine E, Kefauver JM, Liao L, Zhang Y, Gonzalez-Martin A, Shepherd J, Morgan GJ, Mondala TS, Head SR, Kim PH, Xiao N, Fu G, Liu WH, Han J, Williamson JR, Xiao C
PLoS genetics 2017 Feb;13(2):e1006623

Jarid2 binds mono-ubiquitylated H2A lysine 119 to mediate crosstalk between Polycomb complexes PRC1 and PRC2.

Cooper S, Grijzenhout A, Underwood E, Ancelin K, Zhang T, Nesterova TB, Anil-Kirmizitas B, Bassett A, Kooistra SM, Agger K, Helin K, Heard E, Brockdorff N
Nature communications 2016 Nov 28;7:13661

HEB associates with PRC2 and SMAD2/3 to regulate developmental fates.

Yoon SJ, Foley JW, Baker JC
Nature communications 2015 Mar 16;6:6546

JARID2 is involved in transforming growth factor-beta-induced epithelial-mesenchymal transition of lung and colon cancer cell lines.

Tange S, Oktyabri D, Terashima M, Ishimura A, Suzuki T
PloS one 2014;9(12):e115684

Jarid2 is induced by TCR signalling and controls iNKT cell maturation.

Pereira RM, Martinez GJ, Engel I, Cruz-Guilloty F, Barboza BA, Tsagaratou A, Lio CW, Berg LJ, Lee Y, Kronenberg M, Bandukwala HS, Rao A
Nature communications 2014 Aug 8;5:4540

Alu elements in ANRIL non-coding RNA at chromosome 9p21 modulate atherogenic cell functions through trans-regulation of gene networks.

Holdt LM, Hoffmann S, Sass K, Langenberger D, Scholz M, Krohn K, Finstermeier K, Stahringer A, Wilfert W, Beutner F, Gielen S, Schuler G, Gäbel G, Bergert H, Bechmann I, Stadler PF, Thiery J, Teupser D
PLoS genetics 2013;9(7):e1003588

Hira-dependent histone H3.3 deposition facilitates PRC2 recruitment at developmental loci in ES cells.

Banaszynski LA, Wen D, Dewell S, Whitcomb SJ, Lin M, Diaz N, Elsässer SJ, Chapgier A, Goldberg AD, Canaani E, Rafii S, Zheng D, Allis CD
Cell 2013 Sep 26;155(1):107-20

REST-mediated recruitment of polycomb repressor complexes in mammalian cells.

Dietrich N, Lerdrup M, Landt E, Agrawal-Singh S, Bak M, Tommerup N, Rappsilber J, Södersten E, Hansen K
PLoS genetics 2012;8(3):e1002494

A jumonji (Jarid2) protein complex represses cyclin D1 expression by methylation of histone H3-K9.

Shirato H, Ogawa S, Nakajima K, Inagawa M, Kojima M, Tachibana M, Shinkai Y, Takeuchi T
The Journal of biological chemistry 2009 Jan 9;284(2):733-9

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Simple Western: Jumonji/JARID2 Antibody [NB100-2214] - Simple Western lane view shows a specific band for JARID2 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Jumonji/JARID2 Antibody [NB100-2214] - Detection of JARID2 protein in E16 mouse brain lysate using 2ug/ml of NB100-2214.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Jumonji/JARID2 Antibody [NB100-2214] - Jarid2 expression in naive and activated (overnight) murine B cells. This image was submitted via customer Review.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Jumonji/JARID2 Antibody [NB100-2214] - Tested in mouse brain using DAB with hematoxylin counterstain.