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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [2]
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Validation data
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- Product number
- NB500-265 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-265, RRID:AB_2246179
- Product name
- Rabbit Polyclonal DOCK9 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references The immunosenescence-related gene Zizimin2 is associated with early bone marrow B cell development and marginal zone B cell formation.
Age-related guanine nucleotide exchange factor, mouse Zizimin2, induces filopodia in bone marrow-derived dendritic cells.
Matsuda T, Yanase S, Takaoka A, Maruyama M
Immunity & ageing : I & A 2015;12:1
Immunity & ageing : I & A 2015;12:1
Age-related guanine nucleotide exchange factor, mouse Zizimin2, induces filopodia in bone marrow-derived dendritic cells.
Sakabe I, Asai A, Iijima J, Maruyama M
Immunity & ageing : I & A 2012 Apr 11;9:2
Immunity & ageing : I & A 2012 Apr 11;9:2
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DOCK9 Antibody [NB500-265] - Detection of Human and Mouse DOCK9 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DOCK9 antibody NB500-265 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: DOCK9 Antibody [NB500-265] - Detection of Human DOCK9 on HeLa whole cell lystate using NB500-265. DOCK9 was IPed by rabbit anti-DOCK9 antibody NB500-263 and NB500-264 but not by Nb500-265 when using each at 3 mcg/mg lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: DOCK9 Antibody [NB500-265] - Detection of human DOCK9 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DOCK9 antibody NB500-265 (lot NB500-265-2) used for IP at 6 ug per reaction. DOCK9 was also immunoprecipitated by a previous lot of this antibody (lot NB500-265-1) and rabbit anti-DOCK9 antibodies NB500-263 and NB500-264. Detection: Chemiluminescence with an exposure time of 3 minutes.
