Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [7]
- Immunocytochemistry [7]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-20431 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ARMET Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is mouse brain tissue lysate.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse brain tissue lysate using a MANF polyclonal antibody (Product # PA5-20431) at (A) 1 and (B) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ARMET in HEK293T WT or MANF KO cells. Lysates (10 µg) were loaded onto SDS-PAGE and blots were probed with ARMET Polyclonal Antibody (Product # PA5-20431) diluted to 1 µg/mL and anti-beta actin diluted to 1 µg/mL. 1 h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Human Tissues. Loading: 15 µg of lysates per lane. Antibodies: ARMET Polyclonal Antibody (Product # PA5-20431) (2 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Human, Mouse (NIH/3T3) and Rat (YB2/0) Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: ARMET Polyclonal Antibody (Product # PA5-20431) (2 µg /mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: ARMET Polyclonal Antibody (Product # PA5-20431) (2 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Rat Liver. Loading: 15 µg of lysates per lane. Antibodies: ARMET Polyclonal Antibody (Product # PA5-20431) 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 1 µg/mL Lane 2: 2 µg/mL Lane 3: 4 µg/mL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation with Recombinant Protein. Loading: 30 ng of human MANF recombinant protein per lane. Antibodies: ARMET Polyclonal Antibody (Product # PA5-20431) (Lane 1: 0.125 µg/mL, Lane 2: 0.25 µg /mL, and Lane 3: 0.5 µg /mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. Observed at around 55 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of human brain cells using a MANF polyclonal antibody (Product # PA5-20431) at a 20 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed human colon cells labeling MANF with ARMET Polyclonal Antibody (Product # PA5-20431) at 5 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI antibody (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed human cervix cells labeling MANF with ARMET Polyclonal Antibody (Product # PA5-20431) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI antibody (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed human colon cells labeling MANF with ARMET Polyclonal Antibody (Product # PA5-20431) at 5 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI antibody (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed human liver cells labeling MANF with ARMET Polyclonal Antibody (Product # PA5-20431) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI antibody (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse pancreas cells labeling MANF with ARMET Polyclonal Antibody (Product # PA5-20431) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI antibody (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed rat liver cells labeling MANF with ARMET Polyclonal Antibody (Product # PA5-20431) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI antibody (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human brain tissue using ARMET Polyclonal Antibody (Product # PA5-20431) at 2.5 µg /mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.