Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NBP1-91810 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-91810, RRID:AB_11037820
- Product name
- Rabbit Polyclonal CRBN Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human CRBN antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Loss of lenalidomide-induced megakaryocytic differentiation leads to therapy resistance in del(5q) myelodysplastic syndrome.
Cereblon versus VHL: Hijacking E3 ligases against each other using PROTACs.
Iterative Design and Optimization of Initially Inactive Proteolysis Targeting Chimeras (PROTACs) Identify VZ185 as a Potent, Fast, and Selective von Hippel-Lindau (VHL) Based Dual Degrader Probe of BRD9 and BRD7.
Small molecule degraders of the hepatitis C virus protease reduce susceptibility to resistance mutations.
A Chemoproteomic Approach to Query the Degradable Kinome Using a Multi-kinase Degrader.
Pharmacological perturbation of CDK9 using selective CDK9 inhibition or degradation.
Thalidomide promotes degradation of SALL4, a transcription factor implicated in Duane Radial Ray syndrome.
pSILAC mass spectrometry reveals ZFP91 as IMiD-dependent substrate of the CRL4(CRBN) ubiquitin ligase.
Measuring cereblon as a biomarker of response or resistance to lenalidomide and pomalidomide requires use of standardized reagents and understanding of gene complexity.
Martinez-Høyer S, Deng Y, Parker J, Jiang J, Mo A, Docking TR, Gharaee N, Li J, Umlandt P, Fuller M, Jädersten M, Kulasekararaj A, Malcovati L, List AF, Hellström-Lindberg E, Platzbecker U, Karsan A
Nature cell biology 2020 May;22(5):526-533
Nature cell biology 2020 May;22(5):526-533
Cereblon versus VHL: Hijacking E3 ligases against each other using PROTACs.
Girardini M, Maniaci C, Hughes SJ, Testa A, Ciulli A
Bioorganic & medicinal chemistry 2019 Jun 15;27(12):2466-2479
Bioorganic & medicinal chemistry 2019 Jun 15;27(12):2466-2479
Iterative Design and Optimization of Initially Inactive Proteolysis Targeting Chimeras (PROTACs) Identify VZ185 as a Potent, Fast, and Selective von Hippel-Lindau (VHL) Based Dual Degrader Probe of BRD9 and BRD7.
Zoppi V, Hughes SJ, Maniaci C, Testa A, Gmaschitz T, Wieshofer C, Koegl M, Riching KM, Daniels DL, Spallarossa A, Ciulli A
Journal of medicinal chemistry 2019 Jan 24;62(2):699-726
Journal of medicinal chemistry 2019 Jan 24;62(2):699-726
Small molecule degraders of the hepatitis C virus protease reduce susceptibility to resistance mutations.
de Wispelaere M, Du G, Donovan KA, Zhang T, Eleuteri NA, Yuan JC, Kalabathula J, Nowak RP, Fischer ES, Gray NS, Yang PL
Nature communications 2019 Aug 1;10(1):3468
Nature communications 2019 Aug 1;10(1):3468
A Chemoproteomic Approach to Query the Degradable Kinome Using a Multi-kinase Degrader.
Huang HT, Dobrovolsky D, Paulk J, Yang G, Weisberg EL, Doctor ZM, Buckley DL, Cho JH, Ko E, Jang J, Shi K, Choi HG, Griffin JD, Li Y, Treon SP, Fischer ES, Bradner JE, Tan L, Gray NS
Cell chemical biology 2018 Jan 18;25(1):88-99.e6
Cell chemical biology 2018 Jan 18;25(1):88-99.e6
Pharmacological perturbation of CDK9 using selective CDK9 inhibition or degradation.
Olson CM, Jiang B, Erb MA, Liang Y, Doctor ZM, Zhang Z, Zhang T, Kwiatkowski N, Boukhali M, Green JL, Haas W, Nomanbhoy T, Fischer ES, Young RA, Bradner JE, Winter GE, Gray NS
Nature chemical biology 2018 Feb;14(2):163-170
Nature chemical biology 2018 Feb;14(2):163-170
Thalidomide promotes degradation of SALL4, a transcription factor implicated in Duane Radial Ray syndrome.
Donovan KA, An J, Nowak RP, Yuan JC, Fink EC, Berry BC, Ebert BL, Fischer ES
eLife 2018 Aug 1;7
eLife 2018 Aug 1;7
pSILAC mass spectrometry reveals ZFP91 as IMiD-dependent substrate of the CRL4(CRBN) ubiquitin ligase.
An J, Ponthier CM, Sack R, Seebacher J, Stadler MB, Donovan KA, Fischer ES
Nature communications 2017 May 22;8:15398
Nature communications 2017 May 22;8:15398
Measuring cereblon as a biomarker of response or resistance to lenalidomide and pomalidomide requires use of standardized reagents and understanding of gene complexity.
Gandhi AK, Mendy D, Waldman M, Chen G, Rychak E, Miller K, Gaidarova S, Ren Y, Wang M, Breider M, Carmel G, Mahmoudi A, Jackson P, Abbasian M, Cathers BE, Schafer PH, Daniel TO, Lopez-Girona A, Thakurta A, Chopra R
British journal of haematology 2014 Jan;164(2):233-44
British journal of haematology 2014 Jan;164(2):233-44
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: CRBN Antibody [NBP1-91810] - Simple Western lane view shows a specific band for CRBN in 0.2 mg/ml of RT4 (left), U-251MG (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: CRBN Antibody [NBP1-91810] - Electropherogram image(s) of corresponding Simple Western lane view. CRBN antibody was used at 1:60 dilution on RT4 and U-251MG lysate(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CRBN Antibody [NBP1-91810] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CRBN Antibody [NBP1-91810] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CRBN Antibody [NBP1-91810] - Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CRBN Antibody [NBP1-91810] - Immunohistochemical staining of human cerebral cortex shows strong positivity in neuropil.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CRBN Antibody [NBP1-91810] - Staining of human colon shows moderate cytoplasmic and nuclear positivity in peripheral ganglion.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CRBN Antibody [NBP1-91810] - Staining of human skeletal muscle shows only weak cytoplasmic positivity in striated muscle fibers.