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- Antigen structure
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- Western blot [1]
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- Product number
- AF7276 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal Heparan Sulfate Glucosamine 3-O-Sulfotransferase 3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Heparan Sulfate Glucosamine 3-O-Sulfotransferase 3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) HS3ST4, rhHS2ST1, rhHS6ST1, and recombinant mouse HS6ST3 is observed.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references The heparan sulfate 3-O-sulfotransferases (HS3ST) 2, 3B and 4 enhance proliferation and survival in breast cancer MDA-MB-231 cells.
The Pro-Tumoral Activity of Heparan Sulfate 3-O-Sulfotransferase 3B (HS3ST3B) in Breast Cancer MDA-MB-231 Cells Is Dependent on the Expression of Neuropilin-1.
Hellec C, Delos M, Carpentier M, Denys A, Allain F
PloS one 2018;13(3):e0194676
PloS one 2018;13(3):e0194676
The Pro-Tumoral Activity of Heparan Sulfate 3-O-Sulfotransferase 3B (HS3ST3B) in Breast Cancer MDA-MB-231 Cells Is Dependent on the Expression of Neuropilin-1.
Hellec C, Diawara M, Carpentier M, Denys A, Allain F
Molecules (Basel, Switzerland) 2018 Oct 22;23(10)
Molecules (Basel, Switzerland) 2018 Oct 22;23(10)
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human Heparan Sulfate Glucosamine 3-O-Sulfotransferase 3 by Western Blot. Western blot shows lysates of JEG-3 human epithelial choriocarcinoma cell line, JAR human choriocarcinoma cell line, and Capan-1 human pancreatic adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Heparan Sulfate Glucosamine 3-O-Sulfotransferase 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7276) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Heparan Sulfate Glucosamine 3-O-Sulfotransferase 3 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
