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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB10283 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10283, RRID:AB_1676345
- Product name
- NEDD4 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of NEDD4.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references NEDD4-2 (neural precursor cell expressed, developmentally down-regulated 4-2) negatively regulates TGF-beta (transforming growth factor-beta) signalling by inducing ubiquitin-mediated degradation of Smad2 and TGF-beta type I receptor.
Nedd4-like proteins: an emerging family of ubiquitin-protein ligases implicated in diverse cellular functions.
Nedd4 mediates control of an epithelial Na+ channel in salivary duct cells by cytosolic Na+.
Kuratomi G, Komuro A, Goto K, Shinozaki M, Miyazawa K, Miyazono K, Imamura T
The Biochemical journal 2005 Mar 15;386(Pt 3):461-70
The Biochemical journal 2005 Mar 15;386(Pt 3):461-70
Nedd4-like proteins: an emerging family of ubiquitin-protein ligases implicated in diverse cellular functions.
Harvey KF, Kumar S
Trends in cell biology 1999 May;9(5):166-9
Trends in cell biology 1999 May;9(5):166-9
Nedd4 mediates control of an epithelial Na+ channel in salivary duct cells by cytosolic Na+.
Dinudom A, Harvey KF, Komwatana P, Young JA, Kumar S, Cook DI
Proceedings of the National Academy of Sciences of the United States of America 1998 Jun 9;95(12):7169-73
Proceedings of the National Academy of Sciences of the United States of America 1998 Jun 9;95(12):7169-73
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using NEDD4 polyclonal antibody (Cat # PAB10283) shows detection of a 115 kDa band corresponding to endogenous NEDD4 (arrowhead) in MDA-MB-435 cell lysates.The blot was blocked with 5% BLOTTO overnight at 4°C.Primary antibody was used at a 1 : 350 dilution in 5% BLOTTO followed by reaction with a 1 : 20,000 dilution of HRP goat anti-rabbit IgG.ECL was used for detection.
