MAB666-100

antibody from R&D Systems

Targeting: CD226 DNAM-1, DNAM1, PTA1, TLiSA1

Western blot (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB666-100

Provider

R&D Systems

Product name

Human DNAM-1/CD226 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from ascites. Detects human DNAM-1/CD226 in direct ELISAs and Western blots.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

Q15762


Isotype

IgG

Antibody clone number

102511

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Impaired NK cell recognition of vemurafenib-treated melanoma cells is overcome by simultaneous application of histone deacetylase inhibitors.

López-Cobo S, Pieper N, Campos-Silva C, García-Cuesta EM, Reyburn HT, Paschen A, Valés-Gómez M
Oncoimmunology 2018;7(2):e1392426

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The Repeated Administration of Resveratrol Has Measurable Effects on Circulating T-Cell Subsets in Humans.

Espinoza JL, Trung LQ, Inaoka PT, Yamada K, An DT, Mizuno S, Nakao S, Takami A
Oxidative medicine and cellular longevity 2017;2017:6781872

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CD155 on HIV-Infected Cells Is Not Modulated by HIV-1 Vpu and Nef but Synergizes with NKG2D Ligands to Trigger NK Cell Lysis of Autologous Primary HIV-Infected Cells.

Davis ZB, Sowrirajan B, Cogswell A, Ward JP, Planelles V, Barker E
AIDS research and human retroviruses 2017 Feb;33(2):93-100

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Ascorbic acid promotes proliferation of natural killer cell populations in culture systems applicable for natural killer cell therapy.

Huijskens MJ, Walczak M, Sarkar S, Atrafi F, Senden-Gijsbers BL, Tilanus MG, Bos GM, Wieten L, Germeraad WT
Cytotherapy 2015 May;17(5):613-20

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Killing of myeloid APCs via HLA class I, CD2 and CD226 defines a novel mechanism of suppression by human Tr1 cells.

Magnani CF, Alberigo G, Bacchetta R, Serafini G, Andreani M, Roncarolo MG, Gregori S
European journal of immunology 2011 Jun;41(6):1652-62

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of DNAM-1/CD226 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD3^- cells were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB666) or (B) Mouse IgG₁ Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Cell Adhesion Mediated by DNAM-1/CD226 and Neutral-ization by Human DNAM-1/CD226 Antibody. Recombinant Human DNAM-1/CD226 Fc Chimera (Catalog # 666-DN), immobilized onto a microplate, supports the adhesion of the COLO 205 human colorectal adenocarcinoma cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human DNAM-1/CD226 Fc Chimera (20 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB666). The ND₅₀ is typically 0.1-0.6 µg/mL.