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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Other assay [1]
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Validation data
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- Product number
- MA5-15461 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ETV1 Monoclonal Antibody (1C8B6)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15461 targets ETV1 in WB applications and shows reactivity with Human samples.
- Antibody clone number
- 1C8B6
- Concentration
- Conc. Not Determined
Submitted references Plakophilin-3 catenin associates with the ETV1/ER81 transcription factor to positively modulate gene activity.
Munoz WA, Lee M, Miller RK, Ahmed Z, Ji H, Link TM, Lee GR, Kloc M, Ladbury JE, McCrea PD
PloS one 2014;9(1):e86784
PloS one 2014;9(1):e86784
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ETV1 using a ETV1 monoclonal antibody (Product # MA5-15461) against a truncated Trx-ETV1 recombinant protein (1) and full-length ETV1 (aa1-477) human IgG Fc transfected CHO-K1 cell lysate (2).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ETV1 using a ETV1 monoclonal antibody (Product # MA5-15461) against a truncated Trx-ETV1 recombinant protein (1) and full-length ETV1 (aa1-477) human IgG Fc transfected CHO-K1 cell lysate (2).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ETV1 using a ETV1 monoclonal antibody (Product # MA5-15461) against a truncated Trx-ETV1 recombinant protein (1) and full-length ETV1 (aa1-477) human IgG Fc transfected CHO-K1 cell lysate (2).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ETV1 using ETV1 monoclonal antibody (Product # MA5-15461) in HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY ETV1 cDNA (2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 ETV1 is a novel binding partner of Pkp3-catenin. ( A ) Schematics of ETV1 and ETV5 protein structures. Red line indicates the binding region as suggested by sequenced prey clones identified from yeast-two hybrid screen. ( B ) Either HA-Pkp3 or HA-ARVCF was co-transfected with Myc-ETV1 in HEK 293T cells. Anti-Myc (ETV1) or -HA (Pkp3 or ARVCF) immune-precipitates were subjected to immuno-blotting with anti-Myc (ETV1) and -HA (Pkp3 or ARVCF) antibodies. Pkp3-catenin co-precipitates ETV1, but the structurally homologous ARVCF-catenin does not (negative/specificity control). ETV1 co-precipitates Pkp3, but does not co-precipitate ARVCF. ( C ) Untreated AB-1 wild type mouse embryonic stem cells were lysed and fractionated at 90% confluency and endogenous Pkp3 or ETV1 was immuno-precipitated from nuclear extracts, followed by immuno-blotting for Pkp3 and ETV1. Five percent inputs were acquired separately due to the low levels of endogenous proteins.
