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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C773343 - Provider product page
- Provider
- LSBio
- Product name
- FUNDC1 Antibody (PE, Atto 594) LS-C773343
- Antibody type
- Polyclonal
- Description
- Peptide Affinity Purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Red dye
- Storage
- Store at -20°C.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot analysis of Mouse, Rat Liver cell lysates showing detection of ~17.2 kDa FUNDC1 protein using Rabbit Anti-FUNDC1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver cell lysates. Lane 3: Rat Liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-FUNDC1 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~17.2 kDa.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis using Rabbit Anti-FUNDC1 Polyclonal Antibody. Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-FUNDC1 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
