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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NBP2-29951 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal ST8 alpha-2,8-Sialyltransferase 4/ST8SIA4 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.4 ml
- Concentration
- 1.14 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ST8 alpha-2,8-Sialyltransferase 4/ST8SIA4 Antibody [NBP2-29951] - Analysis of ST8SIA4 Antibody (Center) (Cat. #NBP2-29951) in HL-60 cell line lysates (35ug/lane). ST8SIA4 (arrow) was detected using the purified Pab. Western blot analysis of ST8SIA4 Antibody (Center) (NBP2-29951) in mouse spleen tissue lysates (35ug/lane). ST8SIA4 (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ST8 alpha-2,8-Sialyltransferase 4/ST8SIA4 Antibody [NBP2-29951] - IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ST8SIA4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: ST8 alpha-2,8-Sialyltransferase 4/ST8SIA4 Antibody [NBP2-29951] - Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
